Evaluation of biocompatibility of composite biomaterial with anti-inflammatory action, and stimulating tissue recovery process

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Article Title: Evaluation of biocompatibility of composite biomaterial with anti-inflammatory action, and stimulating tissue recovery process
Authors: Iordachel C., Berteanu E., Zuav A.-L., Enache M.-I., Dobre A.-M., Manoiu S., Paraschiv M., Tcacenco L.
Affiliation: Department of Biomaterials & Bioproducts, National Institute R&D for Biological Sciences, Address: 296 Splaiul Independentei, 060031-Bucharest, Romania
Abstract: The paper refers to a bioactive matrix in membrane shape, with a thickness of 0.5 – 1.0 mm, and consisting of gelatine, chitosan, and a-chymotrypsin, with or without addition of glutaraldehyde as a cross-linking agent. The bioproduct is a biomaterial with biomedical applications as biocompatible membrane with anti-inflammatory action due to coupling of the proteolytic enzyme. Bioproducts have complex implications in the treatment of traumatized tissue, accelerating the pus cleansing of infected wound, blood clots and other cellular debris, secretions liquefaction and cleansing of necrotic tissue, and also stimulating tissue regeneration and a faster healing of open infected wounds. In order to establish the influence of crosslinking process and membrane’s thickness on mechanical properties, ultrastructural studies were carried out on obtained biomaterials by electron microscopy analysis. To demonstrate the biological qualities of new enzymatic biomaterials, the membrane’s biological effect was analyzed in a cell culture model (mouse fibroblasts NCTC).
Keywords: biomaterial, chitosan, gelatine, proteolytic enzyme, anti-inflammatory composite.
*Correspondence: Catalin Iordachel, National Institute R&D for Biological Sciences, Department of Biomaterials & Bioproducts, no. 296 Splaiul Independentei, 060031-Bucharest, Romania, Tel./Fax +40 (21)- 220.79.09, e-mail: cataliniordachel@yahoo.com

Cultivation- removed

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Article Title: Cultivation of a miscanthus giganteus on soils polluted with heavy metals and the use of the resulted biomass
Authors: Grama B.G., Pavel B.P., Sand C., Pop M.R., Barbu C.H.
Affiliation: National Institute of Biological Science Research and Development (INCDSB), Bucharest, Romania; Clinical Institute Fundeni, Center of Hematology & Bone Marrow Transplantation ’’St. Berceanu’’, Bucharest; “Vasile Goldis” Western University of Arad, Faculty of Natural Sciences, Arad, Romania
Abstract: The paper presents our results in cultivation on soils polluted with heavy metals (Pb, Cd) of Miscanthus sinensis x giganteus, a sterile hybrid, as well as the tests we have performed for the use of the stems cropped after two years. The results are very encouraging and we expect more and more farmers to cultivate this plant, with benefit for them and for environment.
Keywords: polycythemia vera; PV; rbcs, red blood cells; erythrocyte viability; phosphatidylserine exposure;caspase-3; caspase-8; Annexin-V; Calcein-AM; flow cytometry
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