Researches with reference at polyethylene glycol (PEG) over “in vitro” plants growing

September 24, 2012

Researches with reference at polyethylene glycol (PEG) over “in vitro” plants growing

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Title:	Researches with reference at polyethylene glycol (PEG) over “in vitro” plants growing
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Article_Title:	Researches with reference at polyethylene glycol (PEG) over “in vitro” plants growing
Authors:	Andreea Nistor, Gheorghe Câmpeanu, Nicole Atanasiu, Nicoleta Chiru, Diana Karacsony
Affiliation:	INCDCSZ Braşov, Department of Tissue Culture; USAMV Bucureşti
Abstract:	The MS (Murashige and Skoog 1962) medium was enriched with vitamins, with 20 gl-1 sucrose supplemented with four levels (0.000, 0.006, 0.012 and 0.018 M) of PEG. The pH of the media was adjusted to 5.6-5.8, and media solidified with 8 g/l agar. The media were poured in test tubes, to generate five treatments, three of which were single layer media, with three levels of PEG, respectively, and three were double-layer media with 0.006, 0.012 of PEG, respectively, in the lower layer medium and 0.000 M PEG in the upper layer medium. The varieties studied were Christian and Roclas.
Keywords:	PEG, plantlets, leaves, drought stress, nutrition medium
References:	Abd El-Rahman M.F. Al-Ansary, Aida A. Rizkalla and Awatef M. Badr-Elden, 2007. Micropropagation and Biochemical Genetic Markers detection for Drought and Salt Tolerance of Pear Rootstock. Australian Journal of Basic and Applied Sciences, 1(4): 625-636, 2007 ISSN 1991-8178; Bajji, M., S. Lutts, J. M. Kinet, 2000. Physiological changes after exposure to and recovery from polyethylene glycol-induced water deficit in callus culture issued from durum wheat (Triticum durum) cultivars differing in drought resistance. J. Plant Physiol., 156, 75–83; Boyer J.S. 1982. Plant productivity and environment. Science 218: 443-448; Pertumbuhan Akar Dan Tajuk and Usman K.J. Suharjo, 2007. Use of polyethylene glycol (PEG) 8000 for rapid screening of potato (solanum tuberosum l.) genotypes for water stress tolerance: III. Root and shoot growth. Jurnal Akta Agrosia Edisi Khusus No. 1 hlm 11-18, 2007; Sakthivelu, G., Akitha, Devi, M.K., Giridhar, P., Rajasekaran, T., Ravishankaran, G.,A., Nedev, T., Kosturkova, G., 2008: Drought-induced alterations in growth, osmotic potential and “in vitro” regeneration of soybean cultivar, Gen. Appl. Plant physiology, 2008, special issue, 34 (1-2), 103-112.
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Article Title:	Researches with reference at polyethylene glycol (PEG) over “in vitro” plants growing
Authors:	Andreea Nistor, Gheorghe Câmpeanu, Nicole Atanasiu, Nicoleta Chiru, Diana Karacsony
Affiliation:	INCDCSZ Braşov, Department of Tissue Culture; USAMV Bucureşti
Abstract:	The MS (Murashige and Skoog 1962) medium was enriched with vitamins, with 20 gl-1 sucrose supplemented with four levels (0.000, 0.006, 0.012 and 0.018 M) of PEG. The pH of the media was adjusted to 5.6-5.8, and media solidified with 8 g/l agar. The media were poured in test tubes, to generate five treatments, three of which were single layer media, with three levels of PEG, respectively, and three were double-layer media with 0.006, 0.012 of PEG, respectively, in the lower layer medium and 0.000 M PEG in the upper layer medium. The varieties studied were Christian and Roclas.
Keywords:	PEG, plantlets, leaves, drought stress, nutrition medium
References:	Abd El-Rahman M.F. Al-Ansary, Aida A. Rizkalla and Awatef M. Badr-Elden, 2007. Micropropagation and Biochemical Genetic Markers detection for Drought and Salt Tolerance of Pear Rootstock. Australian Journal of Basic and Applied Sciences, 1(4): 625-636, 2007 ISSN 1991-8178; Bajji, M., S. Lutts, J. M. Kinet, 2000. Physiological changes after exposure to and recovery from polyethylene glycol-induced water deficit in callus culture issued from durum wheat (Triticum durum) cultivars differing in drought resistance. J. Plant Physiol., 156, 75–83; Boyer J.S. 1982. Plant productivity and environment. Science 218: 443-448; Pertumbuhan Akar Dan Tajuk and Usman K.J. Suharjo, 2007. Use of polyethylene glycol (PEG) 8000 for rapid screening of potato (solanum tuberosum l.) genotypes for water stress tolerance: III. Root and shoot growth. Jurnal Akta Agrosia Edisi Khusus No. 1 hlm 11-18, 2007; Sakthivelu, G., Akitha, Devi, M.K., Giridhar, P., Rajasekaran, T., Ravishankaran, G.,A., Nedev, T., Kosturkova, G., 2008: Drought-induced alterations in growth, osmotic potential and “in vitro” regeneration of soybean cultivar, Gen. Appl. Plant physiology, 2008, special issue, 34 (1-2), 103-112.
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