Structural study on the effect of Trigonella foenum graecum seeds on rats’ kidneys

Structural study on the effect of Trigonella foenum graecum seeds on rats’ kidneys

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Title: Structural study on the effect of Trigonella foenum graecum seeds on rats’ kidneys
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Article_Title: Structural study on the effect of Trigonella foenum graecum seeds on rats’ kidneys
Authors: George C. Pribac 1, Aurel Ardelean 1, Constantin Craciun 2, Constantin Puica 2, Corina Rosioru 2, Aurelia Covaci 4, Cecilia Avram 1, Simona Damian 1, Endre Mathe 3, Maria Czapar 3, Lucian Paiusan 1, Liana Mos 1, Coralia Cotoraci 1
Affiliation: 1 “Vasile Goldis” Western University, Faculty of Medicine, Pharmacy and Dental Medicine, Arad, Romania
2 “Babes-Bolyai” University, Biological Research Institute, Cluj-Napoca, Romania
3 University College of Nyiregyhaza, Agrar and Molecular Research Institute, Hungary
4 “Vasile Goldis” Western University, Institute of Life Sciences, Arad, Romania
Abstract: Through their rich content in polyphenolic flavonoids, the Trigonella foenum graecum seeds exert a protective antioxidant effect and membrane protector, certified by the works of some groups of researchers from India, the geographic area where this plant is used not only for its curative effects, but also as a flavoring food supplement. Most researches focused on the hypoglycaemic and antidiabetic action of the seeds, with only a few reports on the use as a preventive factor in the pathology of the alcoholic kidney.
Our work was conducted on an experimental model in vivo; in which the animals were given together with the food two different amounts of grounded seeds, against the background of alcoholic intoxication. The experimental animals were adult Wistar rats, weighing 180-200g, divided into four groups: a witness group (M), which received standard diet and water ad libitum; a group treated with ethanol (ER), administered in the drinking water, 10% (v/v) which received standard diet; two groups that received the same concentration of ethanol, and the food contained 5%, respectively 10% fenugreek flour (T5R and T10R). The ethanol and the flour were administered daily, for 4 weeks.
At structural level, the kidney of the animals from the ER group presented aspects of tubular vascular dystrophy, granular-vascular degeneration and vascular congestions, more evident in the cortical area of the kidneys. The electro-microscopic analysis showed at the level of the proximal convoluted tubes, vacuolation in cytoplasm, edemas at the apical pole of the nephrocytes, rarefaction of the cytoplasmic and mitochondrial matrix, the increase in number of the lysozymes and especially deperoxisomes, as well as the congestion of blood capillaries.
In the case of the groups T5R and T10R, which received Trigonella flour together with ethanol, the structural and ultra-structural changes produced by the ethylic intoxication were more attenuated, being slightly better in group T5R. The vacuolation of the cytoplasm and the number of lysozymes and peroxisomes was greatly reduced, and the aspect of the mitochondria remained in most nephrocytes similar to that of the witness animals. Most of the nuclei of the cells of the nephocytes have retained their spherical shape, being at the same time predominantly euchromatic, with little heterochromatine and evenly dispersed.
Our results plead in favor of using Trigonella seeds as a food supplement to prevent cellular deterioration and improve renal function, together with their already proven hepatoprotective effect (Rosioru et al. 2010; Pribac et al, 2010), in patients suffering from kidney and liver issues caused by excessive alcohol consumption.
Keywords: renal-protective effects – Trigonella seeds – rat kidney – structure and ultra-structure
References: Camelia Ciobanu, A. Ardelean, M. A. Rusu, C. Crăciun,
C. Puică, R. Silaşi, Ioana Roman, L. Barbu-Tudoran,
M. Tămaş, Cercetari histologice, histoenzimatice,
histochimice si electronomicroscopice privind
influenta unui extract bioactiv de Berberis vulgaris
asupra ficatului si rinichiului sobolanilor intoxicati
cu CCl4. Analele SNBC, vol. VIII, cap. 2, 131-158,
2003
Camelia Ciobanu, C. Crăciun, A. Ardelean, M. A. Rusu,
C. Puică, Ioana Roman, L. Barbu-Tudoran, M.
Tămaş, R. Silaşi, Investigatii structural-functionale
privind efectele extractului hidroalcoolic de
Chelidonium majus asupra ficatului si rinichiului
sobolanilor intoxicati cu CCl4. Analele SNBC, vol.
VIII, cap. 2, 104-130, 2003.
Gullo L., Alchool and chronic pancreatitis: leadind or
secondary etiopathogenetic role?. Suppl. 6, 68 –
72, 2005.
Hayat, M.A., Principles and techniques of electron
microscopy – Biological applications, Ed.
Cambridge University Press, Cambridge, 2000
Matsumoto H., Matsumoto I., Alcoholism: protein
expression profiles in human hippocampal model.
Expert Rev. Proteomix 5, 321 – 331, 2008.
Pavelka M., Roth J., Functional ultrastructure. Atlas of tissue biology and pathology. Ed. Springer Wien –
New York, 2005.
Pribac G., Craciun C., Rosioru C., Ardelean A., Czapar M., Simeoni I., Mos L., Ultrastructural studies on hepatoprotective effects of Trigonella foenum graecum seeds in alcoholic rats. Annals of RSCB, vol. XV nr. 2, 373-389, 2010.
Rosioru C., Pribac G., Simeoni I., Craciun C., Ardelean A., Trigonella foenum graecum (Sicklefruit fenugreek) seeds – A natural hepatoprotector that prevents ethanol-induced toxicity. Annals of RSCB, vol. XV nr. 2, 390-399, 2010.
Rusu M. A., Crăciun C., Rusu M. L., Xenobiotice si radicali liberi cu tropism hepatic. Hepatectomia chimica, Ed. Risoprint Cluj-Napoca, 252 pag., ISBN: 973-7833-19-8., 2005.
Tutunaru D., Ciocoiu M., Coman M., Groza. M., Bădescu M., Aspecte morfofuncţionale hepatice în
hepatopatia etanolică. Analele SNBC., XII, 52-56, 2007.
Read_full_article: pdf/21-2011/21-2-2011/SU21-2-2011Pribac.pdf
Correspondence: George Pribac, “Vasile Goldis” Western University Arad, Romania, gpribac@uvvg.ro

Read full article
Article Title: Structural study on the effect of Trigonella foenum graecum seeds on rats’ kidneys
Authors: George C. Pribac 1, Aurel Ardelean 1, Constantin Craciun 2, Constantin Puica 2, Corina Rosioru 2, Aurelia Covaci 4, Cecilia Avram 1, Simona Damian 1, Endre Mathe 3, Maria Czapar 3, Lucian Paiusan 1, Liana Mos 1, Coralia Cotoraci 1
Affiliation: 1 “Vasile Goldis” Western University, Faculty of Medicine, Pharmacy and Dental Medicine, Arad, Romania
2 “Babes-Bolyai” University, Biological Research Institute, Cluj-Napoca, Romania
3 University College of Nyiregyhaza, Agrar and Molecular Research Institute, Hungary
4 “Vasile Goldis” Western University, Institute of Life Sciences, Arad, Romania
Abstract: Through their rich content in polyphenolic flavonoids, the Trigonella foenum graecum seeds exert a protective antioxidant effect and membrane protector, certified by the works of some groups of researchers from India, the geographic area where this plant is used not only for its curative effects, but also as a flavoring food supplement. Most researches focused on the hypoglycaemic and antidiabetic action of the seeds, with only a few reports on the use as a preventive factor in the pathology of the alcoholic kidney.
Our work was conducted on an experimental model in vivo; in which the animals were given together with the food two different amounts of grounded seeds, against the background of alcoholic intoxication. The experimental animals were adult Wistar rats, weighing 180-200g, divided into four groups: a witness group (M), which received standard diet and water ad libitum; a group treated with ethanol (ER), administered in the drinking water, 10% (v/v) which received standard diet; two groups that received the same concentration of ethanol, and the food contained 5%, respectively 10% fenugreek flour (T5R and T10R). The ethanol and the flour were administered daily, for 4 weeks.
At structural level, the kidney of the animals from the ER group presented aspects of tubular vascular dystrophy, granular-vascular degeneration and vascular congestions, more evident in the cortical area of the kidneys. The electro-microscopic analysis showed at the level of the proximal convoluted tubes, vacuolation in cytoplasm, edemas at the apical pole of the nephrocytes, rarefaction of the cytoplasmic and mitochondrial matrix, the increase in number of the lysozymes and especially deperoxisomes, as well as the congestion of blood capillaries.
In the case of the groups T5R and T10R, which received Trigonella flour together with ethanol, the structural and ultra-structural changes produced by the ethylic intoxication were more attenuated, being slightly better in group T5R. The vacuolation of the cytoplasm and the number of lysozymes and peroxisomes was greatly reduced, and the aspect of the mitochondria remained in most nephrocytes similar to that of the witness animals. Most of the nuclei of the cells of the nephocytes have retained their spherical shape, being at the same time predominantly euchromatic, with little heterochromatine and evenly dispersed.
Our results plead in favor of using Trigonella seeds as a food supplement to prevent cellular deterioration and improve renal function, together with their already proven hepatoprotective effect (Rosioru et al. 2010; Pribac et al, 2010), in patients suffering from kidney and liver issues caused by excessive alcohol consumption.
Keywords: renal-protective effects – Trigonella seeds – rat kidney – structure and ultra-structure
References: Camelia Ciobanu, A. Ardelean, M. A. Rusu, C. Crăciun,
C. Puică, R. Silaşi, Ioana Roman, L. Barbu-Tudoran,
M. Tămaş, Cercetari histologice, histoenzimatice,
histochimice si electronomicroscopice privind
influenta unui extract bioactiv de Berberis vulgaris
asupra ficatului si rinichiului sobolanilor intoxicati
cu CCl4. Analele SNBC, vol. VIII, cap. 2, 131-158,
2003
Camelia Ciobanu, C. Crăciun, A. Ardelean, M. A. Rusu,
C. Puică, Ioana Roman, L. Barbu-Tudoran, M.
Tămaş, R. Silaşi, Investigatii structural-functionale
privind efectele extractului hidroalcoolic de
Chelidonium majus asupra ficatului si rinichiului
sobolanilor intoxicati cu CCl4. Analele SNBC, vol.
VIII, cap. 2, 104-130, 2003.
Gullo L., Alchool and chronic pancreatitis: leadind or
secondary etiopathogenetic role?. Suppl. 6, 68 –
72, 2005.
Hayat, M.A., Principles and techniques of electron
microscopy – Biological applications, Ed.
Cambridge University Press, Cambridge, 2000
Matsumoto H., Matsumoto I., Alcoholism: protein
expression profiles in human hippocampal model.
Expert Rev. Proteomix 5, 321 – 331, 2008.
Pavelka M., Roth J., Functional ultrastructure. Atlas of tissue biology and pathology. Ed. Springer Wien –
New York, 2005.
Pribac G., Craciun C., Rosioru C., Ardelean A., Czapar M., Simeoni I., Mos L., Ultrastructural studies on hepatoprotective effects of Trigonella foenum graecum seeds in alcoholic rats. Annals of RSCB, vol. XV nr. 2, 373-389, 2010.
Rosioru C., Pribac G., Simeoni I., Craciun C., Ardelean A., Trigonella foenum graecum (Sicklefruit fenugreek) seeds – A natural hepatoprotector that prevents ethanol-induced toxicity. Annals of RSCB, vol. XV nr. 2, 390-399, 2010.
Rusu M. A., Crăciun C., Rusu M. L., Xenobiotice si radicali liberi cu tropism hepatic. Hepatectomia chimica, Ed. Risoprint Cluj-Napoca, 252 pag., ISBN: 973-7833-19-8., 2005.
Tutunaru D., Ciocoiu M., Coman M., Groza. M., Bădescu M., Aspecte morfofuncţionale hepatice în
hepatopatia etanolică. Analele SNBC., XII, 52-56, 2007.
*Correspondence: George Pribac, “Vasile Goldis” Western University Arad, Romania, gpribac@uvvg.ro