The protective role of N-acetylcysteine in an experimental renal ischemia-reperfusion model on…

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Article Title:	The protective role of N-acetylcysteine in an experimental renal ischemia-reperfusion model on Wistar rats
Authors:	Melania AIRIZER*, Remus MOLDOVAN, Nicoleta DECEA, Adriana MURESAN, Mirela GHERMAN CAPRIOARA
Affiliation:	University of Medicine and Pharmacy ”Iuliu Hatieganu”, Cluj-Napoca, Romania
Abstract:	Many studies have demonstrated that oxidative stress plays an important role in ischemic acute renal failure. The aim of the present study was to investigate the effects of N Acetyl-cystein (ACC) on renal function and the oxidative stress in a rat model of renal ischemia-reperfusion (IR) injury. Histological analysis of kidney tissue was performed. Renal IR was performed by clamping the unilateral renal artery for 20 min followed by 24 h of reperfusion. ACC was administered 72, 24 and 3h prior to IR and the same dose was given 3 h, 24 and 72 h after IR. Treatment groups were: control group, IR group and IR-ACC group. Renal function was assessed by measuring plasma creatinine on days 1, 3 and 7 after IR. Malondialdehyde (MDA) was measured on days 1, 3, 7 after IR. Superoxide dismutase (SOD), catalase and gluthatione were measured as antioxidative markers. IR produced elevated levels of MDA and increased creatinine compared with control group (p<0.05). SOD and catalase levels were lower in IR compared with control group. ACC reduced elevated levels of MDA, increased SOD, catalase and gluthatione levels (P<0.05) and attenuated renal dysfunction. These results demonstrate the antioxidant effect of ACC in attenuating renal IR injury.
Keywords:	ischemia reperfusion model, acetylcystein, oxidative stress, creatinine, histological analysis
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*Correspondence:	Melania Airizer, University of Medicine” Iuliu Hatieganu”,Nephrology Clinic, Cluj Napoca, no. 3-5 Clinicilor St., Fax+40-264-592202, email: melania_airizer@yahoo.com