Analysis of leptin gene expression in severly obese patients

November 17, 2014

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Article Title:	Analysis of leptin gene expression in severly obese patients
Authors:	Kevorkian S.E.M., Hermenean A., Ardelean A., Buburuzan L.
Affiliation:	1″Vasile Goldis” Western University of Arad, Faculty of Medicine, Arad, 2 Department of Biochemistry and Molecular Biology, University of Bucharest,
Abstract:	Obesity is a major health issue in much of the human population. Obesity is a disorder of energy balance, excess energy is stored as fat whenever energy intake exceeds energy expenditure. Leptin is a hormone that regulates body weight by decreasing food intake and increasing energy expenditure. Leptin is expressed in the adipocytes: both its expression and its secretion are highly correlated with body fat and adipocyte size. Circulating leptin concentrations are closely related to adipose tissue. Adipose tissue total RNA from 20 patients with severe obesity was reverse transcribed into complementary DNA, which was used as template for quantitative real-time PCR amplification. The aim of this study was to determine some preliminary information on the level of leptin gene expression in severe obese pacients. It has been found that leptin gene was overexpressed in 19 patients out of 20.
Keywords:	leptin, obesity, Real-Time PCR, humans, GAPDH
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*Correspondence:	Laura Buburuzan, University of Bucharest, Faculty of Biology, Department of Biochemistry and Molecular Biology, no. 91-95 Splaiul Independentei, 050095, Sector 5, Bucharest, Romania, Tel. +40-(021)3181575, email: laura_sv2002@yahoo.com

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