Seed physiological responses in some dicotyledonous plants following liquid nitrogen (-196ºC) exposure

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Article Title:	Seed physiological responses in some dicotyledonous plants following liquid nitrogen (-196ºC) exposure
Authors:	Tamara Moru, Aurel Ardelean
Affiliation:	1 Agricultural Department of Arad, Romania 2 ”Vasile Goldis” Western University Arad, Romania
Abstract:	Seeds cryopreservation is one of the modern methods used to preserve Plant Germplasm Resources. We followed the seed germination of alfalfa, (Medicago sativa) and pea, (Pisum sativum), after seed cryopreservation by immersion in liquid nitrogen (LN) (-196 ºC) for five minutes, one hour, one day, one week and one month. The germination haven’t been affected negatively anyone was the duration of immersion. In case of alfalfa the seed germination increased after seed cryopreservation.
Keywords:	cryopreservation, dicotyledonous plants, alfalfa, pea, germination, seed hardness, abnormal seedlings
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*Correspondence:	Moru Tamara, Agricultural Department of Arad, no. 6A Closca St., tel. 0257-228638, t_m_moru@yahoo.com