Data regarding the morphologic and chemical characterization of Trigonella foenum-graecum seeds

Data regarding the morphologic and chemical characterization of Trigonella foenum-graecum seeds

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Title: Data regarding the morphologic and chemical characterization of Trigonella foenum-graecum seeds
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Article_Title: Data regarding the morphologic and chemical characterization of Trigonella foenum-graecum seeds
Authors: George-Ciprian Pribac, Aurel Ardelean, Violeta Turcus, Claudia-Crina Toma, Coralia Cotoraci, Liana Moș, Constantin Craciun
Affiliation: 1 ”Vasile Goldis” West University Arad, Romania,
2 ”Babes-Bolyai” University, Cluj-Napoca, Romania
Abstract: Trigonella foenum-graecum is an annual, herbal specie, that grows spontaneously on cereals crops and is also known as fenugreek. As powder, the drogue can be identified through chromatography, in the following experiment conditions. Test solution: 1,0g drogue powder mixed with 0.5 ml methanol for 5 minutes in the water bath, heated at 65OC, afterwards chilled and filtrated. Reference solution: 3,0 mg hydrochloric trigonelin dissolved in 1,0 ml methanol. Load: 20 μl test solution and 10 μl reference solution correspond to 2 cm of GF 254 silica gel tape. Solvent system: water – methanol (30 – 70) and migration distance: 10 cm; detection and evaluation: within UV light, with λ=254 nm wavelength. The fenugreek has in its chemical composition 45-60% carbohydrates, especially mucilages located in the call walls; in the endosperm two types of galactomanans are predominant: 1,4-β-glicosil-manose which alternates with α-glicosil-manoze, both connected with a small proportion of xilose. Also, starch and oligo-sacharide fibres are present. As conclusion, even if at least 11 vegetal products were identified, with hypo-cholesterol potential, the proofs remain limited. Along the cholesterol reduction property, some of these plants may have positive aspects for cardio-vascular disorders, by increasing the HDL level and inhibiting the lipid oxidation. The adverse effects of these cures are almost insignificant. Nevertheless, new researches are necessary to establish the therapeutic value of these plants in the hyper-cholesterolemy treatment.
Keywords: Trigonella sp., seeds, morphochemical characterization, diosgenin, trigonellin
References: A. Amin et al. (2005). “Chemopreventive activities of Trigonella foenum graecum (Fenugreek) against
breast cancer”. Cell Biol Int 29 (8): 687-94.
E. Basch et al. (2003). “Therapeutic applications of fenugreek”. Altern Med Rev 8 (1): 20-27.
S. Kaviarasan et al. (2006). “Fenugreek (Trigonella foenum graecum) seed extract prevents ethanol induced toxicity and apoptosis in Chang liver cells”. Alcohol Alcohol 41 (3): 267-273.
J. Raju and R.P. Bird et al. (2006). “Alleviation of hepatic steatosis accompanied by modulation of
plasma and liver TNF-alpha levels by Trigonella foenum graecum (fenugreek) seeds in Zucker obese (fa/fa) rats”. International Journal of Obesity 30 (8): 1298-1307.
J. Raju et al. (2004). “Diosgenin, a steroid saponin of Trigonella foenum graecum (Fenugreek),
inhibits azoxymethane-induced aberrant crypt foci formation in F344 rats and induces
apoptosis in HT-29 human colon cancer cells”. Cancer Epidemiol Biomarkers Prev 13 (8):
1392-1398.
K. Srinivasan et al. (2005). “Plant foods in the management of diabetes mellitus: spices as
beneficial antidiabetic food adjuncts”. International Journal of Food Sciences and Nutrition 56 (6): 399-414.
V. Thirunavukkarasu et al. (2003). “Protective effect of fenugreek (Trigonella foenum graecum) seeds
in experimental ethanol toxicity”. Phytother Res 17 (7): 737-743.
Al Wakeel Sam, Hamed AA, Dadoura SS, Interactive effects of water stress and gibberellic acid an
mineral composition of fenugreek plant. Egyptian Journal of Physiological Sciences, 18
(2): 269-282, 1994.
Brenac P, Sauvaire Y, Chemotaxonomic value of steroLs and steroidal sapogenins in the genus
Trigonella. Biohemical Systetnatics and Ecolog, 24 (2): 157-164, 1996.
Buckeridge MS, Dietrich SMC, Mobilisation of the raffmose family oligosaccharides and
galactomannan in germinating seeds of Sebania marginata Benth. (Leguminosae, Faboideae).
Plant Science, 117 (1-2): 33-43, 1996.
Cerdon C, Rahier A, Taton M, Sauvaire Y, Effect oftridemorph and fenpropimorph on sterol
composition in Fenugreek. Phytochemis-try, 41(2): 423-431, 1996.
Crăciun F, Bojor O, Alexan M, Farmacia Naturii, 1. Edil. Ceres, Bucureşti, 1976.
Ford ES, Giles WH, Dietz WH. Prevalence of the metabolic syndrome among US adults: findings
from the third National Health and Nutrition Examination Survey. JAMA 2002;287:356-359.
Hamed AA, Al Wakeel Sam, Dadoura SS, Interactive effects of water stress and gibberellic acid on
nitrogen content of fenugreek plant. Egyptian Journal of Physiological Sciences, 18 (2): 295-
308, 1994.
Hodişan V, Curs de botanică farmaceutică. înmulţirea plantelor şi sistematică, Ed. a II-a, Litografia UMF Cluj-Napoca, 1993.
Mahna SS, Raisinghani G, Jain, SC, Diosgenin production in induced mutants of Trigonella
corniculata. Fitoterapia, 65 (6): 515-516, 1994.
Mehra P, Kamal R, Effect of fertilizers and foliar sprays on yield and diosgenin content in
fenugreek. Advances in Plant Sciences, 8 (1): 71-77, 1995.
Weder JK, Haussner K, Inhibitors of human and bovine trypsin and chymotrypsin in fenugreek
(Trigonella foenum-graecum L.) seeds. Demonstration and purification. Z Lebensm.
Unters. Forsch., 192 (5): 455-459, 1991.
Yale JF, Prevention of type 2 diabetes. Int J Clin Pract Suppl 2000;113:35-39.
Read_full_article: pdf/18-2008/SU08Pribac.pdf
Correspondence: Pribac George-Ciprian, „Vasile Goldis” West University Arad, Faculty of Medicine, Pharmacy and
Dental Medicine, Department of Cell and Molecular Biology, no. 1 Feleacului St., Arad, +40-257-212204, e-mail:
gpribac@uvvg.ro

Read full article
Article Title: Data regarding the morphologic and chemical characterization of Trigonella foenum-graecum seeds
Authors: George-Ciprian Pribac, Aurel Ardelean, Violeta Turcus, Claudia-Crina Toma, Coralia Cotoraci, Liana Moș, Constantin Craciun
Affiliation: 1 ”Vasile Goldis” West University Arad, Romania,
2 ”Babes-Bolyai” University, Cluj-Napoca, Romania
Abstract: Trigonella foenum-graecum is an annual, herbal specie, that grows spontaneously on cereals crops and is also known as fenugreek. As powder, the drogue can be identified through chromatography, in the following experiment conditions. Test solution: 1,0g drogue powder mixed with 0.5 ml methanol for 5 minutes in the water bath, heated at 65OC, afterwards chilled and filtrated. Reference solution: 3,0 mg hydrochloric trigonelin dissolved in 1,0 ml methanol. Load: 20 μl test solution and 10 μl reference solution correspond to 2 cm of GF 254 silica gel tape. Solvent system: water – methanol (30 – 70) and migration distance: 10 cm; detection and evaluation: within UV light, with λ=254 nm wavelength. The fenugreek has in its chemical composition 45-60% carbohydrates, especially mucilages located in the call walls; in the endosperm two types of galactomanans are predominant: 1,4-β-glicosil-manose which alternates with α-glicosil-manoze, both connected with a small proportion of xilose. Also, starch and oligo-sacharide fibres are present. As conclusion, even if at least 11 vegetal products were identified, with hypo-cholesterol potential, the proofs remain limited. Along the cholesterol reduction property, some of these plants may have positive aspects for cardio-vascular disorders, by increasing the HDL level and inhibiting the lipid oxidation. The adverse effects of these cures are almost insignificant. Nevertheless, new researches are necessary to establish the therapeutic value of these plants in the hyper-cholesterolemy treatment.
Keywords: Trigonella sp., seeds, morphochemical characterization, diosgenin, trigonellin
References: A. Amin et al. (2005). “Chemopreventive activities of Trigonella foenum graecum (Fenugreek) against
breast cancer”. Cell Biol Int 29 (8): 687-94.
E. Basch et al. (2003). “Therapeutic applications of fenugreek”. Altern Med Rev 8 (1): 20-27.
S. Kaviarasan et al. (2006). “Fenugreek (Trigonella foenum graecum) seed extract prevents ethanol induced toxicity and apoptosis in Chang liver cells”. Alcohol Alcohol 41 (3): 267-273.
J. Raju and R.P. Bird et al. (2006). “Alleviation of hepatic steatosis accompanied by modulation of
plasma and liver TNF-alpha levels by Trigonella foenum graecum (fenugreek) seeds in Zucker obese (fa/fa) rats”. International Journal of Obesity 30 (8): 1298-1307.
J. Raju et al. (2004). “Diosgenin, a steroid saponin of Trigonella foenum graecum (Fenugreek),
inhibits azoxymethane-induced aberrant crypt foci formation in F344 rats and induces
apoptosis in HT-29 human colon cancer cells”. Cancer Epidemiol Biomarkers Prev 13 (8):
1392-1398.
K. Srinivasan et al. (2005). “Plant foods in the management of diabetes mellitus: spices as
beneficial antidiabetic food adjuncts”. International Journal of Food Sciences and Nutrition 56 (6): 399-414.
V. Thirunavukkarasu et al. (2003). “Protective effect of fenugreek (Trigonella foenum graecum) seeds
in experimental ethanol toxicity”. Phytother Res 17 (7): 737-743.
Al Wakeel Sam, Hamed AA, Dadoura SS, Interactive effects of water stress and gibberellic acid an
mineral composition of fenugreek plant. Egyptian Journal of Physiological Sciences, 18
(2): 269-282, 1994.
Brenac P, Sauvaire Y, Chemotaxonomic value of steroLs and steroidal sapogenins in the genus
Trigonella. Biohemical Systetnatics and Ecolog, 24 (2): 157-164, 1996.
Buckeridge MS, Dietrich SMC, Mobilisation of the raffmose family oligosaccharides and
galactomannan in germinating seeds of Sebania marginata Benth. (Leguminosae, Faboideae).
Plant Science, 117 (1-2): 33-43, 1996.
Cerdon C, Rahier A, Taton M, Sauvaire Y, Effect oftridemorph and fenpropimorph on sterol
composition in Fenugreek. Phytochemis-try, 41(2): 423-431, 1996.
Crăciun F, Bojor O, Alexan M, Farmacia Naturii, 1. Edil. Ceres, Bucureşti, 1976.
Ford ES, Giles WH, Dietz WH. Prevalence of the metabolic syndrome among US adults: findings
from the third National Health and Nutrition Examination Survey. JAMA 2002;287:356-359.
Hamed AA, Al Wakeel Sam, Dadoura SS, Interactive effects of water stress and gibberellic acid on
nitrogen content of fenugreek plant. Egyptian Journal of Physiological Sciences, 18 (2): 295-
308, 1994.
Hodişan V, Curs de botanică farmaceutică. înmulţirea plantelor şi sistematică, Ed. a II-a, Litografia UMF Cluj-Napoca, 1993.
Mahna SS, Raisinghani G, Jain, SC, Diosgenin production in induced mutants of Trigonella
corniculata. Fitoterapia, 65 (6): 515-516, 1994.
Mehra P, Kamal R, Effect of fertilizers and foliar sprays on yield and diosgenin content in
fenugreek. Advances in Plant Sciences, 8 (1): 71-77, 1995.
Weder JK, Haussner K, Inhibitors of human and bovine trypsin and chymotrypsin in fenugreek
(Trigonella foenum-graecum L.) seeds. Demonstration and purification. Z Lebensm.
Unters. Forsch., 192 (5): 455-459, 1991.
Yale JF, Prevention of type 2 diabetes. Int J Clin Pract Suppl 2000;113:35-39.
*Correspondence: Pribac George-Ciprian, „Vasile Goldis” West University Arad, Faculty of Medicine, Pharmacy and
Dental Medicine, Department of Cell and Molecular Biology, no. 1 Feleacului St., Arad, +40-257-212204, e-mail:
gpribac@uvvg.ro