Peroxidases activity in the germination time of the triticale caryopses after these were cryopreserved
October 11, 2012
Peroxidases activity in the germination time of the triticale caryopses after these were cryopreserved
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Title: | Peroxidases activity in the germination time of the triticale caryopses after these were cryopreserved |
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Article_Title: | Peroxidases activity in the germination time of the triticale caryopses after these were cryopreserved |
Authors: | Monica Şipoş, Dorina Cachiţă-Cosma, Alina Dora Samuel |
Affiliation: | ¹ Departament of Biology, University of Oradea, Romania ² Departament of Biology, “Vasile Goldiş” West University Arad, Romania |
Abstract: | We followed the peroxidases activity (PA) – in first 44 hours of germination – in the exembryonated caryopses of triticale, after grain storage by immersion in liquid nitrogen (LN)(-196ºC) for variable periods of time: 5 minutes, 1 hour, 1 day, 1 week or 1 month. The cryopreservation of the triticale grain for 5 minutes or 1 hour has made that at 6 hours of germination the PA to be significantly decreased in relation with corresponding parameter in the control ex-embryonated caryopses and after 20 and 30 hours from placing the triticale caryopses to germinate decreases did not present statistic relevance; at 44 hours of germination has determined statistically insignificant stimulations of the PA. The cryopreservation of the triticale grains for 1 day in LN has determined – with relation to the control lot – a statistically insignificant inhibition of AP, after 6 and 20 hours of germination; further on, to 30 and 44 hours of germination there have been recorded stimulations of the activity of this enzyme, statistically relevant only at 30 hours from placing the caryopses to germinate. The cryopreservation of the triticale caryopses for 1 week or 1 month has made that at 6 hours of germination the PA activity to be insignificantly decreased in relation with corresponding parameter in the control grains. However, after 20, 30 and 44 hours from placing the triticale caryopses to germinate, stimulations of the PA have been registered; statistical relevance having the stimulations only at 30 hours of germination. |
Keywords: | grains, Triticale, cryopreservation, liquid nitrogen (LN), peroxidases activity (PA) |
References: | Dixit S, Mandal BB, Ahuja S, and Srivastava PS, Genetic stability assessment of plants regenerated from cryopreserved embryogenic tissues of Dioscorea bulbifera L. using RAPD, biochemical and morphological analysis. CryoLetters, 24, pp. 77-84, 2003 Engelmann F, Plant Cryopreservation: progress and prospects. In Vitro Cellular and Development Biology-Plant, 40 (5), pp. 427-433, 2004 Fraignier M.P., Michelle-Ferrière N., Kobrehel K., Distribution of peroxidases in durum wheat (Triticum durum), Cereal Chemistry, 77, pp. 11-17, 2000 Gagliardi RF, Pacheco GP, Carneiro LA, Valls JFM, Vieira MLC, Mansur E, Cryopreservation of Arachis species by vitrification of in vitrogrown shoot apices and genetic stability of recovered plants. CryoLetters, 24, pp. 103-110, 2003 Hornung R., Holland A., Taylor H.F., Lynch P.T., Cryopreservation of Auricula shoot tips using the encapsulation/dehydration technique. CryoLetters, 22, pp. 27-34, 2001 Harding K, Genetic integrity of cryopreserved plant cells: a review. CryoLetters, 25, pp. 3-22, 2004 Lück H., Peroxidaza, In: Methods of Enzymatic Analysis, Ed. Bergmeyer, H.U., Edit. Academic Press New York, pp. 895-897, 1974 Mikula A, Tykarska T, Kuraś M, Ultrastructure of Gentiana tibetica proembryogenic cells before and after cooling treatments. CryoLetters, 26, pp. 367-378, 2005 Steinbach M, Prelucrarea statistică în medicină şi biologie, Edit. Academiei R.P.R., 1961 Touchell D, Walters C, Recovery of embryos of Zizania palustris following exposure to liquid nitrogen. CryoLetters, 21, pp. 261-270, 2000 Zhai Z., Wu Y., Engelmann F., Chen R., and Zhao Y., Genetic stability assessments of plantlets regenerated from cryopreserved in vitro cultured grape and kiwi shoot-tips using RAPD. CryoLetters, 24, pp. 315-322, 2003 |
Read_full_article: | pdf/18-2008/SU08Sipos2.pdf |
Correspondence: | Şipoş M., University of Oradea, Department of Biology, no.1 Universităţii St., Oradea, Romania, email: siposmonica@yahoo.com |
Read full article | |
Article Title: | Peroxidases activity in the germination time of the triticale caryopses after these were cryopreserved |
Authors: | Monica Şipoş, Dorina Cachiţă-Cosma, Alina Dora Samuel |
Affiliation: | ¹ Departament of Biology, University of Oradea, Romania ² Departament of Biology, “Vasile Goldiş” West University Arad, Romania |
Abstract: | We followed the peroxidases activity (PA) – in first 44 hours of germination – in the exembryonated caryopses of triticale, after grain storage by immersion in liquid nitrogen (LN)(-196ºC) for variable periods of time: 5 minutes, 1 hour, 1 day, 1 week or 1 month. The cryopreservation of the triticale grain for 5 minutes or 1 hour has made that at 6 hours of germination the PA to be significantly decreased in relation with corresponding parameter in the control ex-embryonated caryopses and after 20 and 30 hours from placing the triticale caryopses to germinate decreases did not present statistic relevance; at 44 hours of germination has determined statistically insignificant stimulations of the PA. The cryopreservation of the triticale grains for 1 day in LN has determined – with relation to the control lot – a statistically insignificant inhibition of AP, after 6 and 20 hours of germination; further on, to 30 and 44 hours of germination there have been recorded stimulations of the activity of this enzyme, statistically relevant only at 30 hours from placing the caryopses to germinate. The cryopreservation of the triticale caryopses for 1 week or 1 month has made that at 6 hours of germination the PA activity to be insignificantly decreased in relation with corresponding parameter in the control grains. However, after 20, 30 and 44 hours from placing the triticale caryopses to germinate, stimulations of the PA have been registered; statistical relevance having the stimulations only at 30 hours of germination. |
Keywords: | grains, Triticale, cryopreservation, liquid nitrogen (LN), peroxidases activity (PA) |
References: | Dixit S, Mandal BB, Ahuja S, and Srivastava PS, Genetic stability assessment of plants regenerated from cryopreserved embryogenic tissues of Dioscorea bulbifera L. using RAPD, biochemical and morphological analysis. CryoLetters, 24, pp. 77-84, 2003 Engelmann F, Plant Cryopreservation: progress and prospects. In Vitro Cellular and Development Biology-Plant, 40 (5), pp. 427-433, 2004 Fraignier M.P., Michelle-Ferrière N., Kobrehel K., Distribution of peroxidases in durum wheat (Triticum durum), Cereal Chemistry, 77, pp. 11-17, 2000 Gagliardi RF, Pacheco GP, Carneiro LA, Valls JFM, Vieira MLC, Mansur E, Cryopreservation of Arachis species by vitrification of in vitrogrown shoot apices and genetic stability of recovered plants. CryoLetters, 24, pp. 103-110, 2003 Hornung R., Holland A., Taylor H.F., Lynch P.T., Cryopreservation of Auricula shoot tips using the encapsulation/dehydration technique. CryoLetters, 22, pp. 27-34, 2001 Harding K, Genetic integrity of cryopreserved plant cells: a review. CryoLetters, 25, pp. 3-22, 2004 Lück H., Peroxidaza, In: Methods of Enzymatic Analysis, Ed. Bergmeyer, H.U., Edit. Academic Press New York, pp. 895-897, 1974 Mikula A, Tykarska T, Kuraś M, Ultrastructure of Gentiana tibetica proembryogenic cells before and after cooling treatments. CryoLetters, 26, pp. 367-378, 2005 Steinbach M, Prelucrarea statistică în medicină şi biologie, Edit. Academiei R.P.R., 1961 Touchell D, Walters C, Recovery of embryos of Zizania palustris following exposure to liquid nitrogen. CryoLetters, 21, pp. 261-270, 2000 Zhai Z., Wu Y., Engelmann F., Chen R., and Zhao Y., Genetic stability assessments of plantlets regenerated from cryopreserved in vitro cultured grape and kiwi shoot-tips using RAPD. CryoLetters, 24, pp. 315-322, 2003 |
*Correspondence: | Şipoş M., University of Oradea, Department of Biology, no.1 Universităţii St., Oradea, Romania, email: siposmonica@yahoo.com |