Seed physiological responses in some dicotyledonous plants following liquid nitrogen (-196ºC) exposure

October 10, 2012

Seed physiological responses in some dicotyledonous plants following liquid nitrogen (-196ºC) exposure

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Title:	Seed physiological responses in some dicotyledonous plants following liquid nitrogen (-196ºC) exposure
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Article_Title:	Seed physiological responses in some dicotyledonous plants following liquid nitrogen (-196ºC) exposure
Authors:	Tamara Moru, Aurel Ardelean
Affiliation:	1 Agricultural Department of Arad, Romania 2 ”Vasile Goldis” Western University Arad, Romania
Abstract:	Seeds cryopreservation is one of the modern methods used to preserve Plant Germplasm Resources. We followed the seed germination of alfalfa, (Medicago sativa) and pea, (Pisum sativum), after seed cryopreservation by immersion in liquid nitrogen (LN) (-196 ºC) for five minutes, one hour, one day, one week and one month. The germination haven’t been affected negatively anyone was the duration of immersion. In case of alfalfa the seed germination increased after seed cryopreservation.
Keywords:	cryopreservation, dicotyledonous plants, alfalfa, pea, germination, seed hardness, abnormal seedlings
References:	Ardelean A., Mohan Ghe., Botanică sistematică, “Vasile Goldiş” University Press, Arad, pp. 647, 2006 Benson, E.E. Cryopreservation. In E.E. Benson, ed. Plant Conservation Biotechnology. London, England, Taylor & Francis. pp. 83-95, 1999. Brison, M., de Boucaud, M.T., Pierronnet, A. & Dosba, F. Effect of cryopreservation on the sanitary state of a Prunus rootstock experimentally contaminated with Plum Pox Potyvirus. Plant Sci., 123: pp. 189-196, 1997. Cachiţă D., Crăciun C., Cryopreservation of alfalfa and clovers, In Cryopreservation of Plant Germoplasm, vol. 32, Ed.by Y.P.S. BAJAJ, Edit. Springer-Verlag, chap. IV.2, pp.280-286, 1995 Elleuch, H., Gazeau, C., David, H. & David, A., Cryopreservation does not affect the expression of a foreign sam gene in transgenic Papaver somniferum cells.Plant Cell Rep., 18:pp. 94-98, 1998. Engelmann, F. and J.M.M. Engels., Technologies and strategies for ex situ conservation – in Managing Plant Genetic Diversity (J.H.H. Engels, V.R. Rao, A.H.D. Brown and M.T. Jackson, eds.). CAB International, Wallingford, UK/International Plant Genetic Resources Institute, Rome, Italy, pp. 89–104, 2002 Gonzalés-Benito, M.E., Iriondo, J.M., Pita, J.M. & Perez-Garcia, F. Effects of seed cryopreservation and priming on germination in several cultivars of Apium graveolens. Ann. Bot., 75: pp. 1-4, 1995 Kelly KM, Van Staden J, Bell WE., Seed coat structure and dormancy. Plant Growth Regul. 11: pp. 201-209, 1992 Grout, B.W.W. and Crisp, P. Germination as an Unreliable Indicator of the Effectiveness of Cryopreservative Procedures for Imbibed Seeds, Annals ofBotany, London, v.55, pp.289-242, 1985 Helliot, B., Panis, B., PouMay, Y., Swennen, R., Lepoivre, P. & Frison, E.,. Cryopreservation for the elimination of cucumber mosaic and banana streak viruses from banana (Musa spp.). Plant Cell Rep., 20: pp. 1117-1122, 2002 Hong TD, Linington S, Ellis RH, eds. Compendium of information on seed storage behaviour. Rome: International Plant Genetic Resources Institute, 1996 Intenational Seed Testing Association, Don R., ISTA Handbook on Seedling Evaluation, CHSwitzerland, 2003, pp.76- 99 Intenational Seed Testing Association, International Rules for Seed Testing, CH-Switzerland, pp. 65-76, 2006 Lambardi, M., De Carlo, A., Biricolti, S., Puglia, A.M., Lombardo, G., Siragusa, M. & De Pasquale, F., Zygotic and nucellar embryo survival following dehydration/cryopreservation of Citrus intact seeds. CryoLetters, 25: pp. 81-90, 2004 Maxted N., B.V. Ford-Lloyd and J.G. Hawkes., Complementary conservation strategies – in Plant Genetic Resources Conservation (N. Maxted, B.V. Ford-Lloyd and J.G. Hawkes, eds). Chapman & Hall, London1, pp. 15–39, 997 Mazur, P.. Basic concepts in freezing cells. In L.A. Johnson & K. Larsson, eds. Proceedings of the First International Conference on Deep Freezing of Boar Semen, Uppsala, Sweden, pp. 91-111, 1985 Morrison DA, McClay K, Porter C, Rish S., The role of the lens in controlling heat-induced breakdown of testa-imposed dormancy in native Australian legumes. Ann. Bot. 82: pp. 35-40, 1998 Pence, V.C., Cryopreservation of recalcitrant seeds. In Y.P.S. Bajaj, ed. Cryopreservation of plant germplsm I. Biotechnology in agriculture and forestry, vol. 32. Berlin Heidelberg, Germany, Springer. pp. 29-50, 1995 Pence VC ,Germination, desiccation and cryopreservation of seed of Populus deltoides Barr. Seed Sci. Technol. 24: pp. 151-157, 1996 Pritchard HW,Manger KR, Prendergast FG., Changes in Trifolium arvense seed quality following alternating temperature treatment using liquid nitrogen. Annals of Botany 62: pp. 1-11, 1988 Roberts EH., Predicting the storage life of seeds. Seed Science and Technology 1: pp. 499–514, 1973 Seed Science and Technology 1:499–514.Salomão Nassif Antonieta, Tropical seed species’ responses to liquid nitrogen exposure Braz. J. Plant Physiol., 14(2): pp. 133-138, 2002 Wang, Q.C., Marassi, M., Li, P., Gafny, R., Sela, I. , Tanne, E., Elimination of grapevine virus A (GVA) by cryopreservation of in vitro-grown shoot tips of Vitis vinifera L. Plant Sci., 165: pp. 321-327, 2003. www.bioversityinternational.org – Bioversity International, 2006 www.bgci.org – Botanic Gardens Conservation International (BGCI), 2005 www.ipgri.cgiar.org, IPGRI., 2004
Read_full_article:	pdf/18-2008/SU08Moru.pdf
Correspondence:	Moru Tamara, Agricultural Department of Arad, no. 6A Closca St., tel. 0257-228638, t_m_moru@yahoo.com

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Article Title:	Seed physiological responses in some dicotyledonous plants following liquid nitrogen (-196ºC) exposure
Authors:	Tamara Moru, Aurel Ardelean
Affiliation:	1 Agricultural Department of Arad, Romania 2 ”Vasile Goldis” Western University Arad, Romania
Abstract:	Seeds cryopreservation is one of the modern methods used to preserve Plant Germplasm Resources. We followed the seed germination of alfalfa, (Medicago sativa) and pea, (Pisum sativum), after seed cryopreservation by immersion in liquid nitrogen (LN) (-196 ºC) for five minutes, one hour, one day, one week and one month. The germination haven’t been affected negatively anyone was the duration of immersion. In case of alfalfa the seed germination increased after seed cryopreservation.
Keywords:	cryopreservation, dicotyledonous plants, alfalfa, pea, germination, seed hardness, abnormal seedlings
References:	Ardelean A., Mohan Ghe., Botanică sistematică, “Vasile Goldiş” University Press, Arad, pp. 647, 2006 Benson, E.E. Cryopreservation. In E.E. Benson, ed. Plant Conservation Biotechnology. London, England, Taylor & Francis. pp. 83-95, 1999. Brison, M., de Boucaud, M.T., Pierronnet, A. & Dosba, F. Effect of cryopreservation on the sanitary state of a Prunus rootstock experimentally contaminated with Plum Pox Potyvirus. Plant Sci., 123: pp. 189-196, 1997. Cachiţă D., Crăciun C., Cryopreservation of alfalfa and clovers, In Cryopreservation of Plant Germoplasm, vol. 32, Ed.by Y.P.S. BAJAJ, Edit. Springer-Verlag, chap. IV.2, pp.280-286, 1995 Elleuch, H., Gazeau, C., David, H. & David, A., Cryopreservation does not affect the expression of a foreign sam gene in transgenic Papaver somniferum cells.Plant Cell Rep., 18:pp. 94-98, 1998. Engelmann, F. and J.M.M. Engels., Technologies and strategies for ex situ conservation – in Managing Plant Genetic Diversity (J.H.H. Engels, V.R. Rao, A.H.D. Brown and M.T. Jackson, eds.). CAB International, Wallingford, UK/International Plant Genetic Resources Institute, Rome, Italy, pp. 89–104, 2002 Gonzalés-Benito, M.E., Iriondo, J.M., Pita, J.M. & Perez-Garcia, F. Effects of seed cryopreservation and priming on germination in several cultivars of Apium graveolens. Ann. Bot., 75: pp. 1-4, 1995 Kelly KM, Van Staden J, Bell WE., Seed coat structure and dormancy. Plant Growth Regul. 11: pp. 201-209, 1992 Grout, B.W.W. and Crisp, P. Germination as an Unreliable Indicator of the Effectiveness of Cryopreservative Procedures for Imbibed Seeds, Annals ofBotany, London, v.55, pp.289-242, 1985 Helliot, B., Panis, B., PouMay, Y., Swennen, R., Lepoivre, P. & Frison, E.,. Cryopreservation for the elimination of cucumber mosaic and banana streak viruses from banana (Musa spp.). Plant Cell Rep., 20: pp. 1117-1122, 2002 Hong TD, Linington S, Ellis RH, eds. Compendium of information on seed storage behaviour. Rome: International Plant Genetic Resources Institute, 1996 Intenational Seed Testing Association, Don R., ISTA Handbook on Seedling Evaluation, CHSwitzerland, 2003, pp.76- 99 Intenational Seed Testing Association, International Rules for Seed Testing, CH-Switzerland, pp. 65-76, 2006 Lambardi, M., De Carlo, A., Biricolti, S., Puglia, A.M., Lombardo, G., Siragusa, M. & De Pasquale, F., Zygotic and nucellar embryo survival following dehydration/cryopreservation of Citrus intact seeds. CryoLetters, 25: pp. 81-90, 2004 Maxted N., B.V. Ford-Lloyd and J.G. Hawkes., Complementary conservation strategies – in Plant Genetic Resources Conservation (N. Maxted, B.V. Ford-Lloyd and J.G. Hawkes, eds). Chapman & Hall, London1, pp. 15–39, 997 Mazur, P.. Basic concepts in freezing cells. In L.A. Johnson & K. Larsson, eds. Proceedings of the First International Conference on Deep Freezing of Boar Semen, Uppsala, Sweden, pp. 91-111, 1985 Morrison DA, McClay K, Porter C, Rish S., The role of the lens in controlling heat-induced breakdown of testa-imposed dormancy in native Australian legumes. Ann. Bot. 82: pp. 35-40, 1998 Pence, V.C., Cryopreservation of recalcitrant seeds. In Y.P.S. Bajaj, ed. Cryopreservation of plant germplsm I. Biotechnology in agriculture and forestry, vol. 32. Berlin Heidelberg, Germany, Springer. pp. 29-50, 1995 Pence VC ,Germination, desiccation and cryopreservation of seed of Populus deltoides Barr. Seed Sci. Technol. 24: pp. 151-157, 1996 Pritchard HW,Manger KR, Prendergast FG., Changes in Trifolium arvense seed quality following alternating temperature treatment using liquid nitrogen. Annals of Botany 62: pp. 1-11, 1988 Roberts EH., Predicting the storage life of seeds. Seed Science and Technology 1: pp. 499–514, 1973 Seed Science and Technology 1:499–514.Salomão Nassif Antonieta, Tropical seed species’ responses to liquid nitrogen exposure Braz. J. Plant Physiol., 14(2): pp. 133-138, 2002 Wang, Q.C., Marassi, M., Li, P., Gafny, R., Sela, I. , Tanne, E., Elimination of grapevine virus A (GVA) by cryopreservation of in vitro-grown shoot tips of Vitis vinifera L. Plant Sci., 165: pp. 321-327, 2003. www.bioversityinternational.org – Bioversity International, 2006 www.bgci.org – Botanic Gardens Conservation International (BGCI), 2005 www.ipgri.cgiar.org, IPGRI., 2004
*Correspondence:	Moru Tamara, Agricultural Department of Arad, no. 6A Closca St., tel. 0257-228638, t_m_moru@yahoo.com

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