Relation between peritoneal aggression and fibrinolysis
September 17, 2011
Relation between peritoneal aggression and fibrinolysis
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Title: | Relation between peritoneal aggression and fibrinolysis |
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Article_Title: | Relation between peritoneal aggression and fibrinolysis |
Authors: | Sebastian Gradinaru Fometescu1, Costel Savlovschi1, Marieta Costache2, Sorin Mircea Oprescu1 |
Affiliation: | 1 University Emergency Hospital Bucharest, 4th Department of Surgery, Romania 2 University of Bucharest, Department of Biochemistry and Molecular Biology, Romania |
Abstract: | The triggering of the formation of adhesions is caused by the organization of the fibrin matrix, an organization that takes place during the coagulation processes when there is suppression of fibrinolysis. In this study two groups of patients are analyzed First group was used, both for evidencing the peritoneal fibrinolytic activity by determining the fibrinogen and the fibrin degradation products (FDP) in the peritoneal liquid and for extract the tPA and PAI-1 from the peritoneal tissue. The second group is the control group in whose cases none of the aggression factors (traumatic, chemical, bacterial) noticed in the patients of the first group was proven to exist. The aim of this study was to evaluate the role of PAI-1 and tPA genes at peritoneal tissue level. Peritoneal tissue was obtained during surgery and we have quantified the variation of expression of PAI-1 and tPA genes. The obtained results highlight an increase of expression in PAI-1 gene and decrease of expression in tPA gene, which indicated an decrease fibrinolytic potential. |
Keywords: | adhesions, fibrinolysis, fibrin, tPA, PAI-1 |
References: | Cheong YC, Laird SM, Li TC, et al. Peritoneal healing and adhesion formation/reformation. Hum Reprod Update 2001; 7:556-66. Ellis H. The magnitude of adhesion-related problems. Ann Chir Gynaecol 1998;87:9–11. Holmdahl L, Eriksson E, Al-Jabreen M, Risberg B. Fibrinolysis in human peritoneum during operation. Surgery. 1996;119(6):701–705. Imudia AN, Kumar S, Saed GM, Diamond MP. Pathogenesis of Intra-abdominal and pelvic adhesion development. Semin Reprod Med. 2008 Jul;26(4):289-97. Ivarsson M, Holmdahl L, Falk P, et al. Characterisation and fibrinolitic properties of mesothelial cells isolated from peritoneal lavage. Scand J Clin Lab Invest 1998;58: 195-203. Kruithof EKO (1988) Plasminogen activator inhibitors: A review. Enzyme 40:113–121 Menzies D, Ellis H. Intestinal obstruction from adhesions–how big is the problem? Ann R Coll Surg Engl 1990; 72:60-63 Moore KL, Bang NU, Broadie TA, et al. Peritoneal fibrinolysis: evidence for the efficiency of the tissue-type plasminogen activator. J Lab Clin Med 1983;101:921-9. Mutsaers SE, Prêle CM, Lansley SM, Herrick SE. The origin of regenerating mesothelium: a historical perspective. Int J Artif Organs. 2007 Jun;30(6):484-94. Pattinson H, Koninckx P, Brosens I, et al. Clotting and fibrinolytic activities in peritoneal fluid. Br J Obstet Gynecol 1981; 88: 160-6. Rodgers, K.E., and G.S. diZerega. 1993. Function of peritoneal exudate cells after abdominal surgery. J. Invest. Surg. 6:9–23. Rondeau E, Sraer JD, Schleuning WD (1995) The renal plasminogen activating system, in Molecular Nephrology: Kidney Function in Health and Disease, New York, Marcel Decker Inc., pp 699–715 Sawdey MS, Loskuttoff DJ (1991) Regulation of murine type 1 plasminogen activator inhibitor gene expression in vivo. J Clin Invest 88:1346– 1353 Scott-Coombes DM, Whawell SA, Thompson JN. The operative peritoneal fibrinolytic response to abdominal operation. Eur J Surg 1995; 161: 395-399 Sulaiman H, Dawson L, Laurent GJ, et al. Role of plasminogen activators in peritoneal adhesion formation. Biochem Soc Trans 2002;30:126-31. Thompson J, Paterson-Brown S, Harbourne T, et al. Reduced human peritoneal plasminogen activating activity: a possible mechanism of adhesion formation. Br J Surg 1989; 76: 382-84. van Hinsbergh VWM, Kooistra T, Scheffer A, van Bockel JH, van Muijen GNP (1990) Characterization and fibrinolytic properties of human omental tissue mesothelial cells: comparison with endothelial cells. Blood 75:1490 –1497 Vipond MN, Whawell SA, Thompson JN, et al. Peritoneal fibrinolytic activity and intra-abdominal adhesions. Lancet 1990; 335:1120-2. Vipond M, Whawell S, Thompson J, et al. Effect of experimental peritonitis and ischaemia on peritoneal fibrinolytic activity. Eur J Surg 1994; 160:471-77. Vrijland WW, Jeekel J, van Geldorp HJ, et al. Abdominal adhesions: intestinal obstruction, pain, and infertility. Surg Endosc 2003;17:1017-22 Weibel MA, Manjo G. Peritoneal adhesions and their relationship to abdominal surgery. Am J Surg 1973;126: 345-53. |
Read_full_article: | pdf/21-2011/21-1-2011/SU21-1-2011Gradinaru.pdf |
Correspondence: | Marieta Costache, marietacostache@yahoo.com; costache@bio.unibuc.ro |
Read full article | |
Article Title: | Relation between peritoneal aggression and fibrinolysis |
Authors: | Sebastian Gradinaru Fometescu1, Costel Savlovschi1, Marieta Costache2, Sorin Mircea Oprescu1 |
Affiliation: | 1 University Emergency Hospital Bucharest, 4th Department of Surgery, Romania 2 University of Bucharest, Department of Biochemistry and Molecular Biology, Romania |
Abstract: | The triggering of the formation of adhesions is caused by the organization of the fibrin matrix, an organization that takes place during the coagulation processes when there is suppression of fibrinolysis. In this study two groups of patients are analyzed First group was used, both for evidencing the peritoneal fibrinolytic activity by determining the fibrinogen and the fibrin degradation products (FDP) in the peritoneal liquid and for extract the tPA and PAI-1 from the peritoneal tissue. The second group is the control group in whose cases none of the aggression factors (traumatic, chemical, bacterial) noticed in the patients of the first group was proven to exist. The aim of this study was to evaluate the role of PAI-1 and tPA genes at peritoneal tissue level. Peritoneal tissue was obtained during surgery and we have quantified the variation of expression of PAI-1 and tPA genes. The obtained results highlight an increase of expression in PAI-1 gene and decrease of expression in tPA gene, which indicated an decrease fibrinolytic potential. |
Keywords: | adhesions, fibrinolysis, fibrin, tPA, PAI-1 |
References: | Cheong YC, Laird SM, Li TC, et al. Peritoneal healing and adhesion formation/reformation. Hum Reprod Update 2001; 7:556-66. Ellis H. The magnitude of adhesion-related problems. Ann Chir Gynaecol 1998;87:9–11. Holmdahl L, Eriksson E, Al-Jabreen M, Risberg B. Fibrinolysis in human peritoneum during operation. Surgery. 1996;119(6):701–705. Imudia AN, Kumar S, Saed GM, Diamond MP. Pathogenesis of Intra-abdominal and pelvic adhesion development. Semin Reprod Med. 2008 Jul;26(4):289-97. Ivarsson M, Holmdahl L, Falk P, et al. Characterisation and fibrinolitic properties of mesothelial cells isolated from peritoneal lavage. Scand J Clin Lab Invest 1998;58: 195-203. Kruithof EKO (1988) Plasminogen activator inhibitors: A review. Enzyme 40:113–121 Menzies D, Ellis H. Intestinal obstruction from adhesions–how big is the problem? Ann R Coll Surg Engl 1990; 72:60-63 Moore KL, Bang NU, Broadie TA, et al. Peritoneal fibrinolysis: evidence for the efficiency of the tissue-type plasminogen activator. J Lab Clin Med 1983;101:921-9. Mutsaers SE, Prêle CM, Lansley SM, Herrick SE. The origin of regenerating mesothelium: a historical perspective. Int J Artif Organs. 2007 Jun;30(6):484-94. Pattinson H, Koninckx P, Brosens I, et al. Clotting and fibrinolytic activities in peritoneal fluid. Br J Obstet Gynecol 1981; 88: 160-6. Rodgers, K.E., and G.S. diZerega. 1993. Function of peritoneal exudate cells after abdominal surgery. J. Invest. Surg. 6:9–23. Rondeau E, Sraer JD, Schleuning WD (1995) The renal plasminogen activating system, in Molecular Nephrology: Kidney Function in Health and Disease, New York, Marcel Decker Inc., pp 699–715 Sawdey MS, Loskuttoff DJ (1991) Regulation of murine type 1 plasminogen activator inhibitor gene expression in vivo. J Clin Invest 88:1346– 1353 Scott-Coombes DM, Whawell SA, Thompson JN. The operative peritoneal fibrinolytic response to abdominal operation. Eur J Surg 1995; 161: 395-399 Sulaiman H, Dawson L, Laurent GJ, et al. Role of plasminogen activators in peritoneal adhesion formation. Biochem Soc Trans 2002;30:126-31. Thompson J, Paterson-Brown S, Harbourne T, et al. Reduced human peritoneal plasminogen activating activity: a possible mechanism of adhesion formation. Br J Surg 1989; 76: 382-84. van Hinsbergh VWM, Kooistra T, Scheffer A, van Bockel JH, van Muijen GNP (1990) Characterization and fibrinolytic properties of human omental tissue mesothelial cells: comparison with endothelial cells. Blood 75:1490 –1497 Vipond MN, Whawell SA, Thompson JN, et al. Peritoneal fibrinolytic activity and intra-abdominal adhesions. Lancet 1990; 335:1120-2. Vipond M, Whawell S, Thompson J, et al. Effect of experimental peritonitis and ischaemia on peritoneal fibrinolytic activity. Eur J Surg 1994; 160:471-77. Vrijland WW, Jeekel J, van Geldorp HJ, et al. Abdominal adhesions: intestinal obstruction, pain, and infertility. Surg Endosc 2003;17:1017-22 Weibel MA, Manjo G. Peritoneal adhesions and their relationship to abdominal surgery. Am J Surg 1973;126: 345-53. |
*Correspondence: | Marieta Costache, marietacostache@yahoo.com; costache@bio.unibuc.ro |