Thin-layer chromatographic studies of some angiotensin converting enzyme inhibitors and their…

This is an automatically generated default intro template – please do not edit.

	Read full article
Article Title:	Thin-layer chromatographic studies of some angiotensin converting enzyme inhibitors and their inclusion complexes with β-cyclodextrin
Authors:	Laura Sbarcea1*, Lucretia Udrescu1, Liana Dragan1, Cristina Trandafirescu1, Codruta Soica1, Marius Bojita2
Affiliation:	1 “Victor Babes” University of Medicine and Pharmacy, Timisoara, Romania 2 “Iuliu Hatieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania
Abstract:	The aim of this paper is to examine the chromatographic behaviour of some angiotensin converting enzyme (ACE) inhibitors (lisinopril, fosinopril, zofenopril) under conditions of direct phase thin-layer chromatography (Kiselgel 60) and their inclusion complexes with β-cyclodextrin using reversed phase thin-layer chromatography (Kiselgel 60 F254 silaniziert). Binary systems angiotensin converting enzyme inhibitor-β-cyclodextrin were prepared using the kneading method, in 1:1 molar ratio. The thin-layer chromatography reveals that the inclusion complexes show lower hRf values compared to the two components of the inclusion complex, proving that there is an interaction between the active substances and β-cyclodextrin, due to the formation of the inclusion complexes.
Keywords:	ACE-inhibitors, β-cyclodextrin, thin-layer chromatography, inclusion complex, kneading method
References:	Aleksic M, Agbaba D, Baosic R, Milojkovic-Opsenica D, Tesik Z, Thin-layer chromatography of several antihypertensive drugs from the group of angiuotensin converting enzyme inhibitors. J. Serb. Chem. Soc., 2001,66 (1), 39-44 Aleksic M, Agbaba D, Milojkovic-Opsenica D, Tesik Z, Salting-out thin-layer chromatography of some angiotensin converting enzyme inhibitors on different sorbents. Chromatographia Supplement 53, 2001, S-442- S-444 Evangelista S, Manzini S, Antioxidant and cardioprotective properties of sulphydryl angiotensin-converting enzyme inhibitor zofenopril. Journal of international medical research, 2005, 33 (1), 42-54 Ferrar R, Guardigli G, Mele D, Valgimigli M, Ceconi C, Myocardial ischemia: new evidence for angiotensin – converting enzyme inhibition. European Heart Journal Supplements 2003, 5 (Supplement E), E11 – E17 Loftson T, Duchêne D, Cyclodextrins and their pharmaceutical application. Int. J. Pharm., 2007, 329 (1-2), 1-11 Loftson T, Hreinsdottir D, Másson M, Evaluation of cyclodextrin solubilization of drugs. Int. J. Pharm., 2005, 302, 18-28 Odovic J, Stojimirovic B, Aleksic M, Milojkovic-Opsenica D, Tesic Z, Reversed – phase thin – layer chromatography of some angiotensin converting enzyme (ACE) inhibitors and their active metabolites. J. Serb. Chem. Soc., 2006, 71 (6), 621 – 628 Remko M, Acidity, Lipophilicity, Solubility, Absorbtion, and Polar Surface Area of Some ACE Inhibitors. Chem. Pap. 2007, 61 (2) 133-141 Subissi A, Evangelista S, Giachetti A, Preclinical profile of zofenopril: an angiotensin converting enzyme inhibitor with pelicular cardioprotective properties. Cardiovascular Drug Reviews, 1999, 17 (2), 115 – 133 Şoica C, Gyeresi A, Dehelean C, Peev C, Aigner Z, Kata M, Thin-layer chromatography as analytical method for inclusios complexis of some diuretics with cyclodextrins. Farmacia, 2008, vol. LVI, 1, 75-82 Trandafirescu C, Gyéresi Á, Kata M, Aigner Z, Szabadai Z, Studiu privind efectul beta-ciclodextrinei asupra hidrosolubilităţii bifonazolului. Farmacia, 2005, LIII, 5, 83-93 Wang X, Pan J, Shuang S, Thin layer chromatographic study on inclusion complex formation between cyclodextrin and some flavonoids, Analytical letters, 2001, 34 (2), 239-245 *** Farmacopeea Românǎ, Ed a-X-a, Ed Medicala, Bucuresti, 1993 *** European Pharmacopoea, 5th Ed., 2004, Council of Europe, Strasbourg
*Correspondence:	Laura Sbarcea, “Victor Babes” University of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, Eftimie Murgu Str. No. 2, 300041, Timisoara, Romania, email: laura_sbarcea@yahoo.com