Determination of assimilator pigment content in cladodes of Opuntia fragilis var. Fragilis exposed..

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Article Title:	Determination of assimilator pigment content in cladodes of Opuntia fragilis var. Fragilis exposed to light of different colors emitted by LEDs
Authors:	Teodora Iuliana Vidican1, Dorina Cachita-Cosma2
Affiliation:	1 University of Oradea, Romania 2 “Vasile Goldis” Western University of Arad, Romania
Abstract:	After determining the assimilator pigment content of Opuntia fragilis var. fragilis cladodes, from vitrocultures exposed for 90 days at a light intensity of 1000 lux, different colors (white, blue, yellow, red or green), issued by the LED (Light Emitting Diode), it has been found that in relation to their level, similar vitrocultures illuminated with white fluorescent light (control variant, the reference), only Opuntia vitroplantlets exposed to white light emitted by LEDs held chlorophyll a and carotenoid pigments in quantities close to the value determined from material plant derived from control samples. Instead, those vitroplantlets exposed to emitted red or yellow LEDs light, had a content of more than 50% below the lower chlorophyll a and chlorophyll b, in carotenoid pigments respectively, compared with that recorded in similar vitrocladodes from the culture illuminated with white fluorescent tubes (control variant). The strongest inhibitory effect on the assimilator pigment level gained from Opuntia cladodes mesophyll, regenerated in vitro, it has been challenged by green LED light, the vast majority of the examined pigments, the amount of which were approximately 30% from the parameters that have been registered with prepared extracts from control samples.
Keywords:	Opuntia, LEDs, light, pigments, vitrocultures
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*Correspondence:	Teodora Vidican, University of Oradea, Romania, E-mail: teodora.vidican@gmail.com