The occurrence and distribution of adrenomedullin (AM) in the endocrine pancreas of several…

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Article Title:	The occurrence and distribution of adrenomedullin (AM) in the endocrine pancreas of several poikilotherm vertebrates: An immunohistochemical study
Authors:	Ioana Trandaburu1, Tiberiu Trandaburu2
Affiliation:	1 Centre of Cytobiology, Institute of Biology, Bucharest, Romania 2 University of Pitesti, Romania, Faculty of Sciences, Laboratory of Histology and Embryology
Abstract:	The pancreas of six amphibian and two turtle species was investigated for the immunohistochemical detection of the adrenomedullin (AM). The study revealed various degrees of labelling for this neuropeptide depending on the species and endocrine cell type differences, even within the same endocrine cell population. The immunolabelled neural structures represented by nerve fibbers and possible ganglionic perikarya or endocrine cells showed peri-insular localizations in the species Hyla arborea, whereas in the genus Rana were distributed throughout the organ. The AM distribution related to the main insular hormones (PP, SOM, INS, GLUC) was investigated on adjacent serial sections in the species Rana esculenta. The investigations have showed that only part of cells labelled for AM contained also pancreatic hormones in the following order: GLUC > SOM > PP > INS. The above findings and their functional significances supporting the good conservation of AM during phylogeny are discussed in connection with the results previously reported in mammals and other poikilotherm vertebrates.
Keywords:	adrenomedullin, AM, amphibians, turtles, pancreas, immunohistochemistry
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*Correspondence:	Ioana Trandaburu, Institute of Biology, Spl. Independenţei 296, 060031, Bucharest, e-mail : itrandaburu@yahoo.com; ioana.trandaburu@ibiol.ro