Extraction methods comparison for polyphenols recovery and antioxidant activity from Opuntia ficus indica’s seeds

 

 

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Article Title: Extraction methods comparison for polyphenols recovery and antioxidant activity from Opuntia ficus indica’s seeds
Authors: Boudjouan F., Zeghbib W., Bachir Bey M., Djaoudene O., Elothmani D., Bououdina M., Louaileche H.
Affiliation: Laboratoire de Génie de l’Environnement, Faculté de technologie, Université de Bejaia, 06000, Bejaia, Algérie
Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000, Bejaia, Algérie
Laboratoire de Biochimie Appliquée, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000, Bejaia, Algérie
Groupe de Recherche en Agro-alimentaire sur les Produits et les Procédés (ESA), Université d’Angers, Ecole Doctorale : EGAAL « Ecologie, Géosciences, Agronomie et Alimentation », Université d’Angers, Angers, France
Department of Physics, College of Science, University of Bahrain, PO Box 32038, Kingdom of Bahrain
Abstract: The aim of this work is to develop an effective extraction route for biological compounds from Opuntia ficus indica seeds using two conventional methods: water bath shaker and agitation as well as a non-conventional one: microwave. The results indicate a significant effect of both temperature and time on the conventional extraction, with highest values for total phenolic content of 200 and 260 mg Gallic acid equivalent /100 g Dry matter (mg GAE/100 g DM) and for antioxidant activity of 22 and 24 mg GAE/100 g DM, obtained by water bath shaker and stirrer, respectively. However, the microwave extraction method under optimum conditions shows much better extraction efficacy with 300 and 43 mg GAE/100 g DM for total phenolic content and antioxidant activity, respectively. The efficiency of microwave technique could be a promising extraction tool of biological compounds from prickly pears seeds which could eventually be considered as an alternative source for synthetic antioxidants.
Keywords: prickly pear seeds, conventional method, non-conventional method, phenolic compounds, antioxidant activity
*Correspondence: Fares Boudjouan, Laboratoire de Génie de l’Environnement, Faculté de technologie, Université de Bejaia, 06000, Bejaia, Algérie. Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000, Bejaia, Algérie, telephone: 00213 669 328 206, email: fares0501@gmail.com