Flow cytometric analysis of normal and osteoarthritic chondrocytes with lectins

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Article Title:	Flow cytometric analysis of normal and osteoarthritic chondrocytes with lectins
Authors:	Andreea Ciotec, Ana-Maria Dobre, Alexandrina Rugina, Larisa Calu, Ligia Stan, Magdalena Lungu, Nicolae Efimov, Liana Mos, Catalin Iordachel, Daniela Bratosin
Affiliation:	National Institute for Biological Science Research and Development, Bucharest,Romania CFR 2 Hospital, Bucharest, Romania ”Vasle Goldis” Western University of Arad, The Faculty of General Medicine, Pharmacy and Dental Medicine Arad, Romania ”Vasile Goldis” Western University of Arad, Faculty of Natural Sciences, Engineering and Informatics, Arad, Romania
Abstract:	Osteoarthritis (OA) is the most common of all joint diseases, but the molecular basis of its onset and progression is controversial. One characteristic of cartilage is that some chondrocytes reside in areas rich in ECM and it is well known that glycoproteins are abundant on the cell surface and in cartilage ECM. Our working hypothesize was that modification of glycoconjugates contribute to pathogenesis of chondrocyte and we have undertaken a comparative study of normal and OA chondrocytes analysis with fluorescently labeled lectins. Our results clearly indicate that the loss of sialic acid and exposure of the penultimate β-galactose residues of OA chondrocytes glycoconjugates cell surfaces renders cells more susceptible to the membrane damage, which may trigger apoptosis. This could further clarify the relevance of the glycophenotype for chondrocyte function or its significance, if any, as a marker for the development of new chondroprotective agents.
Keywords:	chondrocytes, osteoartritic cartilage, flow cytometric analysis, lectins
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*Correspondence:	Bratosin Daniela, National Institute for Biological Science Research and Development, Bucharest, Romania; Splaiul Independentei no. 296, district 6, Bucharest, Romania, Tel/Fax +40-(021)-2200881, email: bratosind@yahoo.com.