Cellular methods for in vitro engineering of human autologous cartilage. A review

Cellular methods for in vitro engineering of human autologous cartilage. A review

This is an automatically generated default intro template – please do not edit.


General information


Title: Cellular methods for in vitro engineering of human autologous cartilage. A review
Meta keywords:
Meta description:

Images information


Images path absolute: /home/studia/public_html/v15/images/stories/com_form2content/p3/f237
Images path relative: com_form2content/p3/f237
Thumbs path absolute:
Thumbs path relative:

Fields information


Article_Title: Cellular methods for in vitro engineering of human autologous cartilage. A review
Authors: Daniela Bratosin, Catalin Iordachel, Alexandrina Rugina, Ana Maria Gheorghe, Andreea C.I. Ciotec , Nicolae Efimov, Manuela Sidoroff
Affiliation: 1 National Institute for Biological Science Research and Development, Bucharest, Romania
2 ”Vasile Goldis” Western University of Arad, Faculty of Biology, Arad, Romania
3 CFR 2 Hospital, Bucharest, Romania
Abstract: Articular cartilage repair remains a challenge for regenerative medicine. This review summarizes
our research findings that have been undertaken, with a particular emphasis on those techniques that have been introduced into our laboratory, via in vitro and preclinical studies.
Keywords: osteoarthritis, chondrocytes, flow-cytometry, tissue engineering, regenerative medicine
References: Aigner T., Kim H.A., Apoptosis and cellular vitality: Issues in osteoarthritic cartilage degeneration,
Arthritis Rheum., 46:1986-1996, 2002 Aigner T., Kim H.A., H. I. Roach, Apoptosis in osteoarthritis, Rheum. Dis. Clin. N. Am., 30:639-654, 2004
Almarza A.J., Athanasiou K.A., Effects of initial cell seeding density for the tissue engineering of the temporomandibular joint disc. Ann Biomed Eng., 33:943–950, 2005
Aydelotte M.B., Thonar E.J., Mollenhauer J., Flechtenmacher J., Culture of chondrocytes in alginate gel: variations in conditions of gelation influence the structure of the alginate gel, and the arrangement and morphology of proliferating chondrocytes. In Vitro Cell Dev Biol Anim., 34:123–130, 1998
Bratosin D., Estaquier J., Petit F., Arnoult D., Quatannens B., Tissier J.P., Slomianny C., Sartiaux C., Alonso C.,
Huart J.J., Ameisen J.C., Montreuil J., Programmed cell death in mature erythrocytes: a model for
investigating death effector pathways operating in the absence of mitochondria, Cell Death Differ.,
8:1143-1156, 2001
Bratosin D., Buia L., Heron A., Montreuil J., US Patent Application 20100256234, Method of stimulating proliferation of differentiated cells belonging to chondrocytes line”, Published on October 7, 2010
Buckwalter J.A., Lane N.E., Athletics and osteoarthritis. Am. J. Sports Med., 25, 873-881, 1997
Bursch W., Ellinger A., Gerner C., Frohwein U., R. Schulte-Hermann, Programmed cell death (PCD).
Apoptosis, autophagic PCD or others ?, Ann. N.Y. Acad. Sci., 926:1-12, 2001
Buia-Takacs L., Iordachel C., Efimov N., Caloianu M., Montreuil J., Bratosin D., Flow cytometric analysis of encapsulated chondrocytes cultured in alginate gel for cartilage tissue engineering, Jurnal Medical Aradean (Arad Medical Journal), Vol. XIII, issue 4:25-33, 2010
DeLise A.M., Archer C.W., Benjamin M., Ralphs J.R., Organisation of the chondrocyte cytoskeleton and
its response to changing mechanical conditions in organ culture, J. Anat., 194, 343-35, 1999
Fukuda T., Wang H., Nakanishi H., Kosaka K., Yamamoto T., Novel non-apoptotic morphological changes
in neurons of the mouse hippocampus following transient hypoxic-ischemia, Neurosci. Res., 33:49-55, 1999
Gold G.E., Bergman A.G., Pauly J.M., Lang P., Butts R.K., Beaulieu C.F., Hargreaves B., Frank L.,
Boutin R.D., Macovski A., Resnick D., Magnetic resonance imaging of knee cartilage repair. Top
Mag Reson Imag., 9, 377-392, 1998
Grandolfo M., D’andrea P., Paoletti S., Martina M., Silvestrini G., Bonucci E., Vittur F., Culture and differentiation of chondrocytes entrapped in alginate gels. Calcif Tissue Int., 52:42–48, 1993
Guo J.F., Jourdian G.W., Maccallum D.K., Culture and growth characteristics of chondrocytes encapsulated in alginate beads, Connect Tissue Res., 19:277–297, 1989
Hamilton D.W., Riehle M.O., Monaghan W., Curtis A.S., Articular chondrocyte passage number: Influence on adhesion, migration, cytoskeletal organisation and phenotype in response to nano- and micro-metric topography. Cell Biol Int., 29: 408–421, 2005
Kerr J.F.R., Wyllie A.H., Currie A.R., Apoptosis: a basic biological phenomenon with wide-ranging
implications in tissue kinetics, Br. J. Cancer, 26:239- 257,1972
Koch R.J., Gorti G.K., Tissue engineering with chondrocytes. Facial Plast Surg., 18:59–68, 2002
Leist M., Jaattela M., Four death and a funeral: From caspases to alternative mechanisms, Nat. Rev. Mol. Cell Biol., 2:589-598, 2001
Li Y., Ma T., Kniss D.A., Laski L.C., Yang S.T., Effects of filtration seeding on cell density, spatial distribution, and proliferation in nonwoven fibrous matrices. Biotechnol Prog., 17: 935–944, 2001
Liu X., Ma P.X., Polymeric scaffolds for bone tissue engineering. Ann Biomed Eng., 32: 477–486, 2004
Martin J.A., Buckwalter J.A., Aging, articular cartilage chondrocyte senescence and osteoarthritis,
Biogerontolog,. 3, 257-264, 2002
Mcfetridge P.S., Bodamyali T., Horrocks M., Chaudhuri J.B., Endothelial and smooth muscle cell seeding onto processed ex vivo arterial scaffolds using 3D vascular bioreactors. ASAIO J., 50:591–600, 2004
Muir H., The chondrocyte, architect of cartilage. Biomechanics, structure, function and molecular
biology of cartilage matrix macromolecules, Bioessays, 17, 1039-1048, 1995
Paige K.T., Cima L.G., Yaremchuk M.J., Vacanti J.P., Vacanti C.A., Injectable cartilage. Plast Reconstr Surg., 96:1390–1398, 1995
Price J.S., Waters J.G., Darrah C., Pennington C., Edwards D.R., Donell S.T., Clark I.M., The role of chondrocyte senescence in osteoarthritis, 1, 57-65, 2002
Radisic M., Euloth M., Yang L., Langer L.E., Freed G. Vunjak-Novakovic, High-density seeding of myocyte cells for cardiac tissue engineering. Biotechnol Bioeng., 82: 403–414, 2003
Randolph M.A., Anseth K., Yaremchuk M.J., Tissue engineering of cartilage. Clin Plast Surg., 30:519–
537, 2003
Roach H.I., Clarke N.M.P., Cell paralysis as an intermediate stage in the programmed cell death
of epiphyseal chondrocytes, J.Bone Miner Res, 14:1367-1378, 1999
Roach H.I., Clarke N.M.P., Physiological cell death of chondrocytes in vivo is not confined to apoptosis: New observations on the mammalian growth plate, J. Bone Joint Surg., 82-B:601- 613, 2001
Roach H.I., Aigner T., Kouri J.B., Chondroptosis: A variant of apoptotic cell death in chondrocytes ?, Apoptosis, 9:265-27, 2004
Saxon L., Finch C., Bass S., Sports participation, sports injuries and osteoarthritis: Implications for prevention. Sports Med., 28, 123-135, 1999
Shimizu K., Ito A., Honda H., Enhanced cell-seeding into 3D porous scaffolds by use of magnetite
nanoparticles. J Biomed Mater Res B Appl Biomater, 77: 265–272, 2006
Soletti L., Nieponice A., Guan J., Stankus J.J., Wagner W.R., Vorp D.A., A seeding device for tissue
engineered tubular structures. Biomaterials, 27: 4863–4870, 2006
Sperandio, De Belle I., Bredesen D.E., An alternative, nonapoptotic form of programmed cell death., Proc. Natl. Acad. Sci. USA, 97:14376-14381, 2000
Takacs-Buia L., Iordachel C., Efimov N., Caloianu M., Montreuil J., Bratosin D., Pathogenesis of
osteoarthritis: Chondrocyte replicative senescence or apoptosis?, Cytometry Part B (Clinical
Cytometry), 74B:356–362,2008
Ushida T., Furukawa K., Toita K., Tateishi T., Threedimensional seeding of chondrocytes encapsulated
in collagen gel into PLLA scaffolds. Cell Transplant, 11: 489–494, 2002
Vignon E., Arlot M., Patricot I. M., Vignon G., The cell density of human femoral head cartilage, Clin. Orthop., 121:303-308, 1976
Vujak-Novakovic G., Radisic M., Cell seeding of polymer scaffolds. Methods Mol Biol., 238:131–146, 2004
Yang K.G., Saris D.B., Geuze R.E., Van Rijen M.H., Van Der Helm Y.J., Verbout A.J., Creemers L.B., Dhert W.J., Altered in vitro chondrogenic properties of chondrocytes harvested from unaffected cartilage in osteoarthritic joints. Osteoarthritis Cartilage, 14: 561–570, 2006
Yudoh K., Nguyen T., Nakamura H., Hongo-Masuko K., Kato T., Nishioka K., Potential involvement
of oxidative stress in cartilage senescence and development of osteoarthritis: oxidative stress
induces chondrocyte telomere instability and downregulation of chondrocyte function, Arthritis
Res. Ther., 7, 380-391, 2005
Zhao F., Ma T., Perfusion bioreactor system for human mesenchymal stem cell tissue engineering: Dynamic cell seeding and construct development. Biotechnol Bioeng., 91:482–493, 2005
Read_full_article: pdf/21-2011/21-s1-2011/SU21-s1-2011Bratosin3.pdf
Correspondence:

Read full article
Article Title: Cellular methods for in vitro engineering of human autologous cartilage. A review
Authors: Daniela Bratosin, Catalin Iordachel, Alexandrina Rugina, Ana Maria Gheorghe, Andreea C.I. Ciotec , Nicolae Efimov, Manuela Sidoroff
Affiliation: 1 National Institute for Biological Science Research and Development, Bucharest, Romania
2 ”Vasile Goldis” Western University of Arad, Faculty of Biology, Arad, Romania
3 CFR 2 Hospital, Bucharest, Romania
Abstract: Articular cartilage repair remains a challenge for regenerative medicine. This review summarizes
our research findings that have been undertaken, with a particular emphasis on those techniques that have been introduced into our laboratory, via in vitro and preclinical studies.
Keywords: osteoarthritis, chondrocytes, flow-cytometry, tissue engineering, regenerative medicine
References: Aigner T., Kim H.A., Apoptosis and cellular vitality: Issues in osteoarthritic cartilage degeneration,
Arthritis Rheum., 46:1986-1996, 2002 Aigner T., Kim H.A., H. I. Roach, Apoptosis in osteoarthritis, Rheum. Dis. Clin. N. Am., 30:639-654, 2004
Almarza A.J., Athanasiou K.A., Effects of initial cell seeding density for the tissue engineering of the temporomandibular joint disc. Ann Biomed Eng., 33:943–950, 2005
Aydelotte M.B., Thonar E.J., Mollenhauer J., Flechtenmacher J., Culture of chondrocytes in alginate gel: variations in conditions of gelation influence the structure of the alginate gel, and the arrangement and morphology of proliferating chondrocytes. In Vitro Cell Dev Biol Anim., 34:123–130, 1998
Bratosin D., Estaquier J., Petit F., Arnoult D., Quatannens B., Tissier J.P., Slomianny C., Sartiaux C., Alonso C.,
Huart J.J., Ameisen J.C., Montreuil J., Programmed cell death in mature erythrocytes: a model for
investigating death effector pathways operating in the absence of mitochondria, Cell Death Differ.,
8:1143-1156, 2001
Bratosin D., Buia L., Heron A., Montreuil J., US Patent Application 20100256234, Method of stimulating proliferation of differentiated cells belonging to chondrocytes line”, Published on October 7, 2010
Buckwalter J.A., Lane N.E., Athletics and osteoarthritis. Am. J. Sports Med., 25, 873-881, 1997
Bursch W., Ellinger A., Gerner C., Frohwein U., R. Schulte-Hermann, Programmed cell death (PCD).
Apoptosis, autophagic PCD or others ?, Ann. N.Y. Acad. Sci., 926:1-12, 2001
Buia-Takacs L., Iordachel C., Efimov N., Caloianu M., Montreuil J., Bratosin D., Flow cytometric analysis of encapsulated chondrocytes cultured in alginate gel for cartilage tissue engineering, Jurnal Medical Aradean (Arad Medical Journal), Vol. XIII, issue 4:25-33, 2010
DeLise A.M., Archer C.W., Benjamin M., Ralphs J.R., Organisation of the chondrocyte cytoskeleton and
its response to changing mechanical conditions in organ culture, J. Anat., 194, 343-35, 1999
Fukuda T., Wang H., Nakanishi H., Kosaka K., Yamamoto T., Novel non-apoptotic morphological changes
in neurons of the mouse hippocampus following transient hypoxic-ischemia, Neurosci. Res., 33:49-55, 1999
Gold G.E., Bergman A.G., Pauly J.M., Lang P., Butts R.K., Beaulieu C.F., Hargreaves B., Frank L.,
Boutin R.D., Macovski A., Resnick D., Magnetic resonance imaging of knee cartilage repair. Top
Mag Reson Imag., 9, 377-392, 1998
Grandolfo M., D’andrea P., Paoletti S., Martina M., Silvestrini G., Bonucci E., Vittur F., Culture and differentiation of chondrocytes entrapped in alginate gels. Calcif Tissue Int., 52:42–48, 1993
Guo J.F., Jourdian G.W., Maccallum D.K., Culture and growth characteristics of chondrocytes encapsulated in alginate beads, Connect Tissue Res., 19:277–297, 1989
Hamilton D.W., Riehle M.O., Monaghan W., Curtis A.S., Articular chondrocyte passage number: Influence on adhesion, migration, cytoskeletal organisation and phenotype in response to nano- and micro-metric topography. Cell Biol Int., 29: 408–421, 2005
Kerr J.F.R., Wyllie A.H., Currie A.R., Apoptosis: a basic biological phenomenon with wide-ranging
implications in tissue kinetics, Br. J. Cancer, 26:239- 257,1972
Koch R.J., Gorti G.K., Tissue engineering with chondrocytes. Facial Plast Surg., 18:59–68, 2002
Leist M., Jaattela M., Four death and a funeral: From caspases to alternative mechanisms, Nat. Rev. Mol. Cell Biol., 2:589-598, 2001
Li Y., Ma T., Kniss D.A., Laski L.C., Yang S.T., Effects of filtration seeding on cell density, spatial distribution, and proliferation in nonwoven fibrous matrices. Biotechnol Prog., 17: 935–944, 2001
Liu X., Ma P.X., Polymeric scaffolds for bone tissue engineering. Ann Biomed Eng., 32: 477–486, 2004
Martin J.A., Buckwalter J.A., Aging, articular cartilage chondrocyte senescence and osteoarthritis,
Biogerontolog,. 3, 257-264, 2002
Mcfetridge P.S., Bodamyali T., Horrocks M., Chaudhuri J.B., Endothelial and smooth muscle cell seeding onto processed ex vivo arterial scaffolds using 3D vascular bioreactors. ASAIO J., 50:591–600, 2004
Muir H., The chondrocyte, architect of cartilage. Biomechanics, structure, function and molecular
biology of cartilage matrix macromolecules, Bioessays, 17, 1039-1048, 1995
Paige K.T., Cima L.G., Yaremchuk M.J., Vacanti J.P., Vacanti C.A., Injectable cartilage. Plast Reconstr Surg., 96:1390–1398, 1995
Price J.S., Waters J.G., Darrah C., Pennington C., Edwards D.R., Donell S.T., Clark I.M., The role of chondrocyte senescence in osteoarthritis, 1, 57-65, 2002
Radisic M., Euloth M., Yang L., Langer L.E., Freed G. Vunjak-Novakovic, High-density seeding of myocyte cells for cardiac tissue engineering. Biotechnol Bioeng., 82: 403–414, 2003
Randolph M.A., Anseth K., Yaremchuk M.J., Tissue engineering of cartilage. Clin Plast Surg., 30:519–
537, 2003
Roach H.I., Clarke N.M.P., Cell paralysis as an intermediate stage in the programmed cell death
of epiphyseal chondrocytes, J.Bone Miner Res, 14:1367-1378, 1999
Roach H.I., Clarke N.M.P., Physiological cell death of chondrocytes in vivo is not confined to apoptosis: New observations on the mammalian growth plate, J. Bone Joint Surg., 82-B:601- 613, 2001
Roach H.I., Aigner T., Kouri J.B., Chondroptosis: A variant of apoptotic cell death in chondrocytes ?, Apoptosis, 9:265-27, 2004
Saxon L., Finch C., Bass S., Sports participation, sports injuries and osteoarthritis: Implications for prevention. Sports Med., 28, 123-135, 1999
Shimizu K., Ito A., Honda H., Enhanced cell-seeding into 3D porous scaffolds by use of magnetite
nanoparticles. J Biomed Mater Res B Appl Biomater, 77: 265–272, 2006
Soletti L., Nieponice A., Guan J., Stankus J.J., Wagner W.R., Vorp D.A., A seeding device for tissue
engineered tubular structures. Biomaterials, 27: 4863–4870, 2006
Sperandio, De Belle I., Bredesen D.E., An alternative, nonapoptotic form of programmed cell death., Proc. Natl. Acad. Sci. USA, 97:14376-14381, 2000
Takacs-Buia L., Iordachel C., Efimov N., Caloianu M., Montreuil J., Bratosin D., Pathogenesis of
osteoarthritis: Chondrocyte replicative senescence or apoptosis?, Cytometry Part B (Clinical
Cytometry), 74B:356–362,2008
Ushida T., Furukawa K., Toita K., Tateishi T., Threedimensional seeding of chondrocytes encapsulated
in collagen gel into PLLA scaffolds. Cell Transplant, 11: 489–494, 2002
Vignon E., Arlot M., Patricot I. M., Vignon G., The cell density of human femoral head cartilage, Clin. Orthop., 121:303-308, 1976
Vujak-Novakovic G., Radisic M., Cell seeding of polymer scaffolds. Methods Mol Biol., 238:131–146, 2004
Yang K.G., Saris D.B., Geuze R.E., Van Rijen M.H., Van Der Helm Y.J., Verbout A.J., Creemers L.B., Dhert W.J., Altered in vitro chondrogenic properties of chondrocytes harvested from unaffected cartilage in osteoarthritic joints. Osteoarthritis Cartilage, 14: 561–570, 2006
Yudoh K., Nguyen T., Nakamura H., Hongo-Masuko K., Kato T., Nishioka K., Potential involvement
of oxidative stress in cartilage senescence and development of osteoarthritis: oxidative stress
induces chondrocyte telomere instability and downregulation of chondrocyte function, Arthritis
Res. Ther., 7, 380-391, 2005
Zhao F., Ma T., Perfusion bioreactor system for human mesenchymal stem cell tissue engineering: Dynamic cell seeding and construct development. Biotechnol Bioeng., 91:482–493, 2005
*Correspondence: