The cytological effects of chlorhexidine compounds with copper ion tested on Allium root meristem

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Article Title:	The cytological effects of chlorhexidine compounds with copper ion tested on Allium root meristem
Authors:	Verginica Schröder, Ticuța Negreanu-Pîrjol, Mariana Arcuș, Gabriela Lilios, Gheorghe Țarălungă
Affiliation:	Department of Cell and Molecular Biology, Department of Chemistry, Department of Botany, Department of Pathophysiology, Department of Pharmacology , Faculty of Pharmacy, Ovidius University Constanta, Romania
Abstract:	The chlorhexidine complexes with metallic ions of copper are newly synthesized in order to obtain topical pharmaceutical preparations with antiseptic and anti-inflammatory activities, but without adverse effects or allergic reactions on the mucous membranes or epithelia. The testing of the cytological activities on the radix meristem of Allium cepa completes the previous tests accomplished on microorganism. Bulbs (2 cm in diameter), with 5-10 mm radices, were introduced into solutions of the complexes. The used complexes have ethanol (10-3) as solvent, therefore the control samples were: water, ethanol (250 μL, 500 μL) in order to record possible influences of the solvent volume that could cover the real effects of the compound. The radices were measured and then the meristems were harvested at 24 hours and 96 hours respectively. The microscope preparations were fixed in acetic acid 45% and then colored with Schiff reagent. The results emphasized that all the compounds have a cytostatic effect, but do not manifest genotoxicity.
Keywords:	cytostatic, cytotoxic, mitotic index, root cells
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*Correspondence:	Schröder Verginica,Ovidius University Constanta, Faculty of Pharmacy, Department of Cell and Molecular, Biology.No. 18 Garofitei Street, Constanta, Romania, e-mail: virgischroder@yahoo.com