Mediums quality and their ability to support developing of preimplanted mouse embryos

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Article Title:	Mediums quality and their ability to support developing of preimplanted mouse embryos
Authors:	Svetlana KOGĂLNICEANU-ODOBESCU¹, Ioana LĂNCRĂJAN¹, Doru Ardelean¹, Ioan VINTILĂ²
Affiliation:	¹„Vasile Goldiş” Western University of Arad, Faculty of Life Sciences, Romania ² University of Agricultural Sciences and Veterinary Medicine, Timişoara, Romania
Abstract:	Embrionary development begins with the fertilization, after the formation of zygote. Our aim was the study some factors (temperature, media, atmosphere) that can influence the development of preimplantation embryo by different species and humans. The influence of culture medium in which the embryos were grown was under research regarding the transfer of embryos from in vivo medium to in vitro culture media. We investigated methods for developing culture media and determining requirements for development in vitro. Inactived rat serum and addition of protein (FCS), on defined culture medium TCM 199, rise the efficiency of growing method, and exhibit higher ability to support blastocysts developing until eclosing from zona prellucida as the correspondingly defined media.
Keywords:	embryos, media, preimplantation, fertilization, in vitro culture
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*Correspondence:	Svetlana Kogălniceanu-Odobescu, „Vasile Goldiş” Western University of Arad, Faculty of Life Sciences, email: sos_kg@yahoo.com