Effects of dirt treatments on the enzyme activities of the soil in Sikfokut site

Effects of dirt treatments on the enzyme activities of the soil in Sikfokut site

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Title: Effects of dirt treatments on the enzyme activities of the soil in Sikfokut site
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Article_Title: Effects of dirt treatments on the enzyme activities of the soil in Sikfokut site
Authors: Istvan FEKETE1, Zsolt KOTROCZÓ2, Csaba VARGA3, Zsuzsa VERES4, Zsolt KRAKOMPERGER4, János Attila TÓTH4
Affiliation: 1College of Nyíregyháza, Institute of Environmental Science, Hungary
2College of Nyíregyháza, Institute of Biology; Hungary
3College of Nyíregyháza, Department of Land and Environmental Management, Hungary
4University of Debrecen, Department of Ecology; Hungary
Abstract: The soil organic matter is affected among others by the quality of litter input i.e. the kind of litter material that is deposited on soil surface. Leaf, root and wood litter addition were applied and the effect of these treatments on soil enzymes activities (polyphenoloxidase, saccharase and phosphatase). The rapidly decomposing leaf and root litter had a great influence on enzyme activities. First the decomposition of fine roots affected in the case of polyphenoloxidase and phosphatase activity. The investigations of phenoloxidase and phosphatase which were carried out right after the establishment of parcels, showed absolutely higher values in soil samples of NI and NR treatments in the first years. Then the leaf litter decomposition was the most important influencing factor. Later these values decreased and was exceeded by the DL (Double Litter), C (Control), DW (Double Wood) treatments, in which surface leaf-litter was added to the soil. The wood litter decomposition did not affect the polyphenoloxidase activity substantially during the experimental period. The phosphatase activity showed the same tendency as polyphenoloxidase, but wood litter played a greater role in phosphatase activity, and the activity declined rapidly in the case of root treatments. The examination of the saccharase activity was launched three and a half years after the establishment of the plots; thus having enough time for the development of the different ecological conditions at our disposal. Significant differences were detectable between the treatments in the case of saccharase and phosphatase.
Keywords: polyphenoloxidase, phosphatase, saccharase, soil enzyme activity, litter decomposition, DIRT treatments
References: Anderson M., Kjøller A., Struwe S., Microbial enzyme activities in leaf litter, humus and mineral soil layers of European forests. Soil Biology & Biochemistry, 36: 1527-1537, 2004.
Antal E., Berki I., Justyák J., Kiss Gy., Tarr K., Vig P., A síkfőkúti erdőtársulás hő- és vízháztartási viszonyainak vizsgálata az erdőpusztulás és az éghajlatváltozás tükrében, Debrecen, 83 p, 1997.
Burns R. G., Enzyme activity in soil: location and a possible role in microbial ecology. Soil Biol. Biochem. 14:423-427, 1982.
Fekete, I., Varga, Cs., Kotroczó, Zs., Krakomperger, Zs., Tóth, J. A., The effect of temperature and moisture on enzyme activity in Síkfőkút Site. Cereal Research Communications Volume 35, Issue 2, 381-385, 2007.
Frankenberger W. T., Johanson J. B., Method of measuring invertase activity in soils. Plant and Soil 74: 313-323, 1983.
Freeman C., Ostle N., Kang H., An enzymic ‘latch’ on a global carbon store – A shortage of oxygen locks up carbon in peatlands by restraining a single enzyme. Nature, 409: 149, 2001.
Harrison A. F., Pearce T., Seasonal variation of phosphatase activity in woodland soils. Soil Biol. Biochem. 11: 405-410, 1979.
Kotroczó, Zs., Fekete, I., Tóth J. A., Tóthmérész B., Balázsy S., Effect of leaf and root-litter manipulation for carbon-dioxide efflux in forest soil. Cer. Res. Comm. Volume Suppl. 36: 663-666 pp., 2008.
Krakomperger Zs, Tóth J. A., Varga Cs., Tóthmérész B., The effect of litter input on soil enzyme activity in an oak forest. Cereal Research Communications Vol. 36. 323-326, 2008.
Larson J.L., Zak D.R., Sinsabaugh R.L. Extracellular enzyme activity beneath temperate trees growing under elevated carbon dioxide and ozone. Soil Science Society of America Journal, 66: 1848- 1856., 2002.
Nadelhoffer K., Boone, R., Bowden, R., Canary, J., Kaye, J., Micks, P., Ricca, A., McDowell, W., Aitkenhead, J. The DIRT experiment. In: Foster, D. R., Aber, D. J. (eds) Forests in Time. Yale Univ. Press, Michigan, 2004.
Nielson G.A., Hole F.D., A study of the natural processes of incorporation of organic matter into soil in the University of Wisconsin Arboretum. Wisconsin Academic Review, 52:231-227, 1963.
Qualls R.G., Haines B.L., Swank W 332 .T., Fluxes of dissolved organic nutrients and humic substances in a deciduous fores. Ecology, 72: 254-266, 1991.
Sinsabaugh R. L. – Klug M. J. – Collins H.P. – Yeager P.E. – Petersen S.O., Characterizing Soil Microbioal Communities. In: Robertson G.P. – Coleman D.C.– Bledsoe C.S. – Sollins P. (ed): Standard Soil Methods for Long-term ecological research. Oxford University Press 318-348, 1999.
Sinsabaugh R.L., Enzymic analysis of microbial pattern and process – Biol. Fertil. Soils, 17: 69-74, 1994.
Sinsabaugh R.L., Antibus R.K., Linkins A.E., McClaugherty C.A., Rayburn L., Repert D., Weiland T., Wood decomposition: Nitrogen and phosphorus dynamics in relation to extracellular enzyme activity. Ecology, 74: 1586-1693, 1993.
Sinsabaugh R.L., Carrerio M.M., Repert, D.A., Allocation of extracellular enzymatic activity in relation to litter composition, N deposition, and mass loss. Biogeochemistry, 60: 1-24, 2002.
Sowerby A., Emmett B., Beier C., Tietema A., Penuelas J. Estiarrte M., Meeteren J. M., Hughes S., Freeman C., Microbial community changes in heathland soil communities along a geographical gradient: interaction with climate change manipulations. Soil Biology & Biochemistry, 37:1805-1813, 2005.
Tarafdar J. C., Jungk A., Phosphatase activity in the rhizosphere and its relation to the depletion of soil organic phosphorus. Biol. Fertil. Soils, 3: 199-204, 1987.
Tóth, J. A., K. Lajtha, Zs. Kotroczó, Zs. Krakomperger, B. Caldwel, R. D. Bowden, M. Papp, The effect of climate change on soil organic matter decomposition. Acta Silvatica et Ligniaria Hungarica Vol. 3: 75-85. pp., 2007.
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Correspondence: István Fekete Ph.D Collage of Nyíregyháza, Institute of Environmental Science, H-4400 Nyíregyháza, Sóstói u. 31./b. Phone: +36 42 599-400 ext. 2294, Fax: + 36 42 402-485, E-mail: feketeistani@gmail.com

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Article Title: Effects of dirt treatments on the enzyme activities of the soil in Sikfokut site
Authors: Istvan FEKETE1, Zsolt KOTROCZÓ2, Csaba VARGA3, Zsuzsa VERES4, Zsolt KRAKOMPERGER4, János Attila TÓTH4
Affiliation: 1College of Nyíregyháza, Institute of Environmental Science, Hungary
2College of Nyíregyháza, Institute of Biology; Hungary
3College of Nyíregyháza, Department of Land and Environmental Management, Hungary
4University of Debrecen, Department of Ecology; Hungary
Abstract: The soil organic matter is affected among others by the quality of litter input i.e. the kind of litter material that is deposited on soil surface. Leaf, root and wood litter addition were applied and the effect of these treatments on soil enzymes activities (polyphenoloxidase, saccharase and phosphatase). The rapidly decomposing leaf and root litter had a great influence on enzyme activities. First the decomposition of fine roots affected in the case of polyphenoloxidase and phosphatase activity. The investigations of phenoloxidase and phosphatase which were carried out right after the establishment of parcels, showed absolutely higher values in soil samples of NI and NR treatments in the first years. Then the leaf litter decomposition was the most important influencing factor. Later these values decreased and was exceeded by the DL (Double Litter), C (Control), DW (Double Wood) treatments, in which surface leaf-litter was added to the soil. The wood litter decomposition did not affect the polyphenoloxidase activity substantially during the experimental period. The phosphatase activity showed the same tendency as polyphenoloxidase, but wood litter played a greater role in phosphatase activity, and the activity declined rapidly in the case of root treatments. The examination of the saccharase activity was launched three and a half years after the establishment of the plots; thus having enough time for the development of the different ecological conditions at our disposal. Significant differences were detectable between the treatments in the case of saccharase and phosphatase.
Keywords: polyphenoloxidase, phosphatase, saccharase, soil enzyme activity, litter decomposition, DIRT treatments
References Anderson M., Kjøller A., Struwe S., Microbial enzyme activities in leaf litter, humus and mineral soil layers of European forests. Soil Biology & Biochemistry, 36: 1527-1537, 2004.
Antal E., Berki I., Justyák J., Kiss Gy., Tarr K., Vig P., A síkfőkúti erdőtársulás hő- és vízháztartási viszonyainak vizsgálata az erdőpusztulás és az éghajlatváltozás tükrében, Debrecen, 83 p, 1997.
Burns R. G., Enzyme activity in soil: location and a possible role in microbial ecology. Soil Biol. Biochem. 14:423-427, 1982.
Fekete, I., Varga, Cs., Kotroczó, Zs., Krakomperger, Zs., Tóth, J. A., The effect of temperature and moisture on enzyme activity in Síkfőkút Site. Cereal Research Communications Volume 35, Issue 2, 381-385, 2007.
Frankenberger W. T., Johanson J. B., Method of measuring invertase activity in soils. Plant and Soil 74: 313-323, 1983.
Freeman C., Ostle N., Kang H., An enzymic ‘latch’ on a global carbon store – A shortage of oxygen locks up carbon in peatlands by restraining a single enzyme. Nature, 409: 149, 2001.
Harrison A. F., Pearce T., Seasonal variation of phosphatase activity in woodland soils. Soil Biol. Biochem. 11: 405-410, 1979.
Kotroczó, Zs., Fekete, I., Tóth J. A., Tóthmérész B., Balázsy S., Effect of leaf and root-litter manipulation for carbon-dioxide efflux in forest soil. Cer. Res. Comm. Volume Suppl. 36: 663-666 pp., 2008.
Krakomperger Zs, Tóth J. A., Varga Cs., Tóthmérész B., The effect of litter input on soil enzyme activity in an oak forest. Cereal Research Communications Vol. 36. 323-326, 2008.
Larson J.L., Zak D.R., Sinsabaugh R.L. Extracellular enzyme activity beneath temperate trees growing under elevated carbon dioxide and ozone. Soil Science Society of America Journal, 66: 1848-1856., 2002.
Nadelhoffer K., Boone, R., Bowden, R., Canary, J., Kaye, J., Micks, P., Ricca, A., McDowell, W., Aitkenhead, J. The DIRT experiment. In: Foster, D. R., Aber, D. J. (eds) Forests in Time. Yale Univ. Press, Michigan, 2004.
Nielson G.A., Hole F.D., A study of the natural processes of incorporation of organic matter into soil in the University of Wisconsin Arboretum. Wisconsin Academic Review, 52:231-227, 1963.
Qualls R.G., Haines B.L., Swank W 332 .T., Fluxes of dissolved organic nutrients and humic substances in a deciduous fores. Ecology, 72: 254-266, 1991.
Sinsabaugh R. L. – Klug M. J. – Collins H.P. – Yeager P.E. – Petersen S.O., Characterizing Soil Microbioal Communities. In: Robertson G.P. – Coleman D.C. – Bledsoe C.S. – Sollins P. (ed): Standard Soil Methods for Long-term ecological research. Oxford University Press 318-348, 1999.
Sinsabaugh R.L., Enzymic analysis of microbial pattern and process – Biol. Fertil. Soils, 17: 69-74, 1994.
Sinsabaugh R.L., Antibus R.K., Linkins A.E., McClaugherty C.A., Rayburn L., Repert D., Weiland T., Wood decomposition: Nitrogen and phosphorus dynamics in relation to extracellular enzyme activity. Ecology, 74: 1586-1693, 1993.
Sinsabaugh R.L., Carrerio M.M., Repert, D.A., Allocation of extracellular enzymatic activity in relation to litter composition, N deposition, and mass loss. Biogeochemistry, 60: 1-24, 2002.
Sowerby A., Emmett B., Beier C., Tietema A., Penuelas J. Estiarrte M., Meeteren J. M., Hughes S., Freeman C., Microbial community changes in heathland soil communities along a geographical gradient: interaction with climate change manipulations. Soil Biology & Biochemistry, 37:1805-1813, 2005.
Tarafdar J. C., Jungk A., Phosphatase activity in the rhizosphere and its relation to the depletion of soil organic phosphorus. Biol. Fertil. Soils, 3: 199-204, 1987.
Tóth, J. A., K. Lajtha, Zs. Kotroczó, Zs. Krakomperger, B. Caldwel, R. D. Bowden, M. Papp, The effect of climate change on soil organic matter decomposition. Acta Silvatica et Ligniaria Hungarica Vol. 3: 75-85. pp., 2007.
*Correspondence: István Fekete Ph.D Collage of Nyíregyháza, Institute of Environmental Science, H-4400 Nyíregyháza, Sóstói u. 31./b. Phone: +36 42 599-400 ext. 2294, Fax: + 36 42 402-485, E-mail: feketeistani@gmail.com