Flow cytometric analysis as a quick and efficient method for morphological changes determination…

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Article Title:	Flow cytometric analysis as a quick and efficient method for morphological changes determination in hematological diseases. RBCs of Gaucher disease as a model
Authors:	Ana-Maria Gheorghe1, Cyril Mignot2, Coralia Cotoraci3, Aurel Ardelean4, Jean Montreuil5, Daniela Bratosin1,4*
Affiliation:	1 National Institute for Biological Science Research and Development, Bucharest, Romania 2 Reference Center for Lysosomal Storage Disorders, Assistance Publique – Hôpitaux de Paris, Armand Trousseau Hospital, Department of Neuropediatrics, Paris, France 3 ″Vasile Goldis″ Western University of Arad, Faculty of Medicine, Arad, Romania 4 ″Vasile Goldis″ Western University of Arad, Faculty of Natural Sciences, Arad, Romania 5 Université des Sciences et Technologies de Lille 1, Laboratoire de Chimie Biologique, UMR CNRS/USTL 8576, Villeneuve d’Ascq, France
Abstract:	Flow cytometry is a general method for the rapid individual analysis of a large numbers of cells using light-scattering, fluorescence and absorbance measurements. The forward-scattered light provides informations about the size of the cells and can be detected without further manipulation. The sideways-scattered light is affected by several parameters, including granularity, cell size and cell morphology. Gaucher disease (GD) is a sphingolipidosis caused by a deficiency of the enzyme glucocerebrosidase and enhanced erythrophagocytosis is one feature of the disease indicating abnormal macrophage-RBC interactions. The aim of this study was to compare flow cytometric analysis with the morphology obtained by scanning electron microscopy analysis (SEM) of RBCs sampled from seven untreated type 1 GD patients and two of the same patients who then underwent nine months of enzyme replacement therapy (ERT). The results provide that flow cytometric analysis can be a quick and efficient method in haematological diseases diagnosis and monitoring.
Keywords:	flow cytometry, scanning electron microscopy, erythrocytes, Gaucher disease, morphological changes, medical monitoring
References:	Bitton A, Etzell J, Grenert JP, Wang E. Erythrophagocytosis in Gaucher cells. Arch Pathol Lab Med 2004;128(10):1191-2. Métézeau P, Ronot X., Le Noan-Merdrignac G., Ratinaud M.H., La Cytométrie en flux pour l’étude de la cellule normale ou pathologique, Medsi/mcgraw-Hill, Paris, 1988. Mignot C, Doummar D, Maire I, De Villemeur TB. Type 2 Gaucher disease: 15 new cases and review of the literature. Brain Dev 2006;28(1):39-48. Montreuil J, Boulanger P, Houcke E. Chromatographie sur papier des constituants glucidiques des cérébrosides d’une rate de Gaucher. Bull Sté Chim Biol 1953;35(10):1125-1127. Ronot X., Grunwald D., Mayol J-F., Boutonnat J., La cytométrie en flux, Lavoisier, Paris, 2006. Sharpe LR, Ancliff P, Amrolia P, Gilmour KC, Vellodi A. Type II Gaucher disease manifesting as haemophagocytic lymphohistiocytosis. J Inherit Metab Dis 2009.
*Correspondence:	Daniela Bratosin, National Institute for Biological Science Research and Development, Spl. Independentei nº 296, Bucharest, and ″Vasile Goldis″ Western University of Arad, Faculty of Natural Sciences, Arad, Romania, Tel/Fax: 40.21.2200881; E-mail: bratosind@yahoo.com