Comparative karyotype in different lineages of cyprinid fish (Teleostei: Cypriniformes: Cyprinidae)

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Article Title:	Comparative karyotype in different lineages of cyprinid fish (Teleostei: Cypriniformes: Cyprinidae)
Authors:	Catalina LUCA1, Radu SUCIU2, Marieta COSTACHE1
Affiliation:	1 Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, Romania 2 Danube Delta National Institute for Research and Development, Tulcea, Romania
Abstract:	The analysis of the karyotype was performed through the investigation of the number and structure of the cyprinid fish species chromosomes. The fish used in this study were caught in the Danube River. For the subsequent analysis of karyotypes, the fish were injected intra-peritoneal with doses of 0.02 ml/g body weight of 0.1% colchicine solution and left for 180 min before sacrificing. The karyotype conservation of the Romanian genera Leuciscinae and Barbinae subfamilies was determined by metaphase investigation. The study also emphasized that the Abramis bjoerka and Scardinius erythrophtalmus are phylogenetically close, belonging to the Leuciscinae lineage, while the Barbus barbus species belongs to a different group of cyprinids, the Barbinae lineage.
Keywords:	karyotype, cytotaxonomy of cyprinids, Scardinius erythrophthalmus, Abramis bjoerka, Barbus barbus
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*Correspondence:	Luca C., University of Bucharest, Faculty of Biology, Biochemistry and Molecular Biology Department, 91-95 Spl. Independentei, Bucharest 5, Romania; email: catalina2006luca@gmail.com