Studies regarding the effects of salicylic acid on maize (Zea mays L.) seedling under salt stress

Studies regarding the effects of salicylic acid on maize (Zea mays L.) seedling under salt stress

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Title: Studies regarding the effects of salicylic acid on maize (Zea mays L.) seedling under salt stress
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Article_Title: Studies regarding the effects of salicylic acid on maize (Zea mays L.) seedling under salt stress
Authors: Cornelia PURCĂREA1*, Dorina CACHIŢĂ-COSMA2
Affiliation: 1 Faculty of Environmental Protection, University of Oradea, Romania
2 “Vasile Goldis” Western University of Arad, Romania
Abstract: Abiotic stresses such as heavy metals, salinity, drought, temperature, UV-radiation, ozone, cause drastic yield reduction in most crops. Plants have complex and dynamic systems of response to stress. Salt stress affects around 20% of the world’s cultivated areas. Salicylic acid (SA) plays an important role in response to biotic and abiotic stress. Pre-treatment of maize seeds with SA may cause a low level of oxidative stress, improving the antioxidative capacity of the plants. Salicylic acid can increase the plant tolerance to salt stress induced in our experiment by 150 mM NaCl treatments. In our experiment we determined the effect of pretreatment of maize seeds with 0.5 mM concentration of salicylic acid (SA) solution on growth, peroxidase activity, assimilatory pigments and protein content of the 3thd leaves of maize seedlings under salt stress. The results obtained showed that exogenous application of SA induced an increase in growth parameters of maize seedlings and the peroxidase activity in the roots of maize seedlings was lower than in untreated plants. The treatment with SA ameliorates the total clorophyllian pigment content and the content of total soluble protein in maize seedling leaves under salt stress.
Keywords: maize, salicylic acid, growth, peroxidase activity, protein content, assimilatory pigments
References: Arberg B. – Plant growth regulators. Monosubstituted benzoic acid, ,Sweed. Agric.Res., 11, p. 93-105, 1981.
Barkosky RR., Einhellig FA., Effects of salicylic acid on plant water relationship, Journal of Chemical Ecology, 19, p 237-247, 1993.
Borsani O.,Valpuesta V., Botlleb M.A., Evidence for a role of salicylic acid in the oxidative damage generated by NaCl and osmotic stress in Arabidobsis seedlings. Plant Physiology 126, p.1024-1030, 2001.
Bradford M.M., -A rapid and sensitive method for the quantitation of microgram quantities of protein-dye binding, Anal. Biochem. 72, p.248-254, 1976.
Deef H.E., – Influence of Salicylic acid on stress tolerance during seed germination of Triticum aestivum and Hordeum vulgare, Advances in Biological Research 1 (1-2): p.40-48, 2007.
Hans-Luck, Methdos of Enzymatic Analysis Verlag Chemie Gmb H. (Ed).Weinheim Bergstr. Academic Press, New york and London.p.885-888, 1970.
Hayat S., Ahmad A., Salicylic acid: a plant hormone, Springer (ed) dortrecht, the Netherlands, 2007.
Hayat S., Fariduddin Q., Ali, B., and Ahmad A., Effects of salicylic acid on growth and enzymes activities of wheat caryopsislings, Acta.agron.hung., 53, p.433-437, 2005.
Hussein M.M., Balbaa L.K., and Gaballah M.S., Salicylic Acid and Salinity Effects on Growth of Maize Plants;
Research Journal of Agriculture and Biological Sciences, 3(4), p. 321-328, 2007.
Janda T., Szalai G., Tari I., Páldi E., (1999) – Hydroponic treatment with salicylic acid decreases the effect of chilling injury in maize (Zea mays) plants. Planta 208, p.175-180.
Khan W., Prithiviraj B, Smith DL, Photosynthetic responses of corn and soybean to foliar application of salicylates, Journal of plant physiology, 160, p.485-492, 2003.
Moharekar ST., Lokhande SD, Hara, T., Tanaka, R., Tanaka, A., and Chavan PD., Effect of salicylic acid on clorophyll and carotenoid contents of wheat and moong caryopsislings, Photosynthetica, 41,p. 315-317, 2003.
Moran R, Porath D, Clorophyll determination in intact tissue using N,N- dimethylformamide, Plant physiol, 65, p.478-479, 1980.
Moran R., Formulae for determination of chlorophyllous pigments extracted with N,N- dimethylformamide, Plant physiol. 69 (6), p.1376-1381, 1982.
Nafees K. A.; S., Sarvajeet, 2007, Abiotic Stress and Plant Responses, New Delhi, India: Narosa Publishing House.
Purcǎrea C., Cachiţǎ-Cosma D., – Comparative studies about the influence of salicylic and acetylsalicilic acid on content of assimilatory pigments in the primary leaves of sunflower (Helianthus sp.) plantlets. Arad, May 2008, “Studia Universitatis Vasile Goldis, Arad”, Seria Stiinţele vieţii, vol.18, ISSN: 1584-2363, pp. p. 51-54, 2008. (a)
Purcǎrea C., Cachiţǎ-Cosma D., – The influence of salicylic acid and acetylsalicylic acid on the growth of sunflower (Helianthus sp.) seedling roots and on their total absorption capacity. – Arad, May 2008, “Studia Universitatis Vasile Goldis, Arad”, Seria Stiinţele vieţii, vol.18. ISSN: 1584-2363, p. 55-60, 2008 (b).
Quiroz-Figueroa F., Mendez-Zeel M., Larque-Saavedra. Loyola-Vargas, VM., Picomolar salicylate levels enhance cell growth and embryogenesis, Plant cell reports 20, p 679-684, 2001.
Ryang S.Z., Woo S.Y., Kwon S.Y., Kim S.H., Lee S.H., Kim K.N., Lee D.K., Changes of net photosynthesis, antioxidant enzyme activities, and antioxidant contents of Liriodendron tulipifera under elevated ozone. Photosynthetica. 47, p.19-25.2009.
Shakirova F.M., Sakhabudinova A.R., Bezrukova M.V., Fakhutdinova R. A., Fakhutdinova D.R.– Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity. Plant. Sci. 164, p.317-322, 2003.
Senaratna, T., D. Tuochell, T. Bunn and K. Dixon. Acetylsalicylic acid (aspirin) and Salicylic acid induce multiple
stress tolerance in bean and tomato plants. Plant Growth Regul., 30, p.157-161. 2000.
Szepesi, Á., J. Csiszár, S. Bajkán, K. Gémes and F. Horvath. Role of Salicylic acid pre-treatment on the acclimation of tomato plants to salt- and osmotic stress. Acta Biologica Szegediensis, 49 p.123-125, 2005
Tari, I., J. Csiszár, G. Szalai, F. Horváth, A. Pécsváradi, G. Kiss, Á. Szepesi, M. Szabó and L. Erdei. Acclimation of tomato plants to salinity stress after a Salicylic acid pre-treatement, Acta Biol. Szeged, 46, p.55-56, 2002.
Tari, I., Simon L.M., Deer K.J., . Csiszár, J., Bajkan Sz., Kis Gy., and Szepesi, Á. Influence of salicylic acid on salt stress acclimation of tomato plants: oxidative stress response and osmotic adaptation. Acta Physiol. Plant, p.268:237, 2004.
Tasgin E., Atici O., Nalbantoglu B. – Effects of salicylic acid and cold on freezing tolerance in winter wheat leaves. Plant Growth Regul. 41, p. 231-236, 2003.
Read_full_article: pdf/20-2010/20-1-2010/SU20-1-10Purcarea2.pdf
Correspondence: Cornelia Purcărea, University of Oradea, Faculty of Environmental Protection, No. 26 Gen. Magheru St., 410048, Oradea, Romania, email cpurcarea@uoradea.ro

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Article Title: Studies regarding the effects of salicylic acid on maize (Zea mays L.) seedling under salt stress
Authors: Cornelia PURCĂREA1*, Dorina CACHIŢĂ-COSMA2
Affiliation: 1 Faculty of Environmental Protection, University of Oradea, Romania
2 “Vasile Goldis” Western University of Arad, Romania
Abstract: Abiotic stresses such as heavy metals, salinity, drought, temperature, UV-radiation, ozone, cause drastic yield reduction in most crops. Plants have complex and dynamic systems of response to stress. Salt stress affects around 20% of the world’s cultivated areas. Salicylic acid (SA) plays an important role in response to biotic and abiotic stress. Pre-treatment of maize seeds with SA may cause a low level of oxidative stress, improving the antioxidative capacity of the plants. Salicylic acid can increase the plant tolerance to salt stress induced in our experiment by 150 mM NaCl treatments. In our experiment we determined the effect of pretreatment of maize seeds with 0.5 mM concentration of salicylic acid (SA) solution on growth, peroxidase activity, assimilatory pigments and protein content of the 3thd leaves of maize seedlings under salt stress. The results obtained showed that exogenous application of SA induced an increase in growth parameters of maize seedlings and the peroxidase activity in the roots of maize seedlings was lower than in untreated plants. The treatment with SA ameliorates the total clorophyllian pigment content and the content of total soluble protein in maize seedling leaves under salt stress.
Keywords: maize, salicylic acid, growth, peroxidase activity, protein content, assimilatory pigments
References: Arberg B. – Plant growth regulators. Monosubstituted benzoic acid, ,Sweed. Agric.Res., 11, p. 93-105, 1981.
Barkosky RR., Einhellig FA., Effects of salicylic acid on plant water relationship, Journal of Chemical Ecology, 19, p 237-247, 1993.
Borsani O.,Valpuesta V., Botlleb M.A., Evidence for a role of salicylic acid in the oxidative damage generated by NaCl and osmotic stress in Arabidobsis seedlings. Plant Physiology 126, p.1024-1030, 2001.
Bradford M.M., -A rapid and sensitive method for the quantitation of microgram quantities of protein-dye binding, Anal. Biochem. 72, p.248-254, 1976.
Deef H.E., – Influence of Salicylic acid on stress tolerance during seed germination of Triticum aestivum and Hordeum vulgare, Advances in Biological Research 1 (1-2): p.40-48, 2007.
Hans-Luck, Methdos of Enzymatic Analysis Verlag Chemie Gmb H. (Ed).Weinheim Bergstr. Academic Press, New york and London.p.885-888, 1970.
Hayat S., Ahmad A., Salicylic acid: a plant hormone, Springer (ed) dortrecht, the Netherlands, 2007.
Hayat S., Fariduddin Q., Ali, B., and Ahmad A., Effects of salicylic acid on growth and enzymes activities of wheat caryopsislings, Acta.agron.hung., 53, p.433-437, 2005.
Hussein M.M., Balbaa L.K., and Gaballah M.S., Salicylic Acid and Salinity Effects on Growth of Maize Plants;
Research Journal of Agriculture and Biological Sciences, 3(4), p. 321-328, 2007.
Janda T., Szalai G., Tari I., Páldi E., (1999) – Hydroponic treatment with salicylic acid decreases the effect of chilling injury in maize (Zea mays) plants. Planta 208, p.175-180.
Khan W., Prithiviraj B, Smith DL, Photosynthetic responses of corn and soybean to foliar application of salicylates, Journal of plant physiology, 160, p.485-492, 2003.
Moharekar ST., Lokhande SD, Hara, T., Tanaka, R., Tanaka, A., and Chavan PD., Effect of salicylic acid on clorophyll and carotenoid contents of wheat and moong caryopsislings, Photosynthetica, 41,p. 315-317, 2003.
Moran R, Porath D, Clorophyll determination in intact tissue using N,N- dimethylformamide, Plant physiol, 65, p.478-479, 1980.
Moran R., Formulae for determination of chlorophyllous pigments extracted with N,N- dimethylformamide, Plant physiol. 69 (6), p.1376-1381, 1982.
Nafees K. A.; S., Sarvajeet, 2007, Abiotic Stress and Plant Responses, New Delhi, India: Narosa Publishing House.
Purcǎrea C., Cachiţǎ-Cosma D., – Comparative studies about the influence of salicylic and acetylsalicilic acid on content of assimilatory pigments in the primary leaves of sunflower (Helianthus sp.) plantlets. Arad, May 2008, “Studia Universitatis Vasile Goldis, Arad”, Seria Stiinţele vieţii, vol.18, ISSN: 1584-2363, pp. p. 51-54, 2008. (a)
Purcǎrea C., Cachiţǎ-Cosma D., – The influence of salicylic acid and acetylsalicylic acid on the growth of sunflower (Helianthus sp.) seedling roots and on their total absorption capacity. – Arad, May 2008, “Studia Universitatis Vasile Goldis, Arad”, Seria Stiinţele vieţii, vol.18. ISSN: 1584-2363, p. 55-60, 2008 (b).
Quiroz-Figueroa F., Mendez-Zeel M., Larque-Saavedra. Loyola-Vargas, VM., Picomolar salicylate levels enhance cell growth and embryogenesis, Plant cell reports 20, p 679-684, 2001.
Ryang S.Z., Woo S.Y., Kwon S.Y., Kim S.H., Lee S.H., Kim K.N., Lee D.K., Changes of net photosynthesis, antioxidant enzyme activities, and antioxidant contents of Liriodendron tulipifera under elevated ozone. Photosynthetica. 47, p.19-25.2009.
Shakirova F.M., Sakhabudinova A.R., Bezrukova M.V., Fakhutdinova R. A., Fakhutdinova D.R.– Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity. Plant. Sci. 164, p.317-322, 2003.
Senaratna, T., D. Tuochell, T. Bunn and K. Dixon. Acetylsalicylic acid (aspirin) and Salicylic acid induce multiple
stress tolerance in bean and tomato plants. Plant Growth Regul., 30, p.157-161. 2000.
Szepesi, Á., J. Csiszár, S. Bajkán, K. Gémes and F. Horvath. Role of Salicylic acid pre-treatment on the acclimation of tomato plants to salt- and osmotic stress. Acta Biologica Szegediensis, 49 p.123-125, 2005
Tari, I., J. Csiszár, G. Szalai, F. Horváth, A. Pécsváradi, G. Kiss, Á. Szepesi, M. Szabó and L. Erdei. Acclimation of tomato plants to salinity stress after a Salicylic acid pre-treatement, Acta Biol. Szeged, 46, p.55-56, 2002.
Tari, I., Simon L.M., Deer K.J., . Csiszár, J., Bajkan Sz., Kis Gy., and Szepesi, Á. Influence of salicylic acid on salt stress acclimation of tomato plants: oxidative stress response and osmotic adaptation. Acta Physiol. Plant, p.268:237, 2004.
Tasgin E., Atici O., Nalbantoglu B. – Effects of salicylic acid and cold on freezing tolerance in winter wheat leaves. Plant Growth Regul. 41, p. 231-236, 2003.
*Correspondence: Cornelia Purcărea, University of Oradea, Faculty of Environmental Protection, No. 26 Gen. Magheru St., 410048, Oradea, Romania, email cpurcarea@uoradea.ro