CagA phosphorylation in Helicobacter pylori infection

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Article Title:	CagA phosphorylation in Helicobacter pylori infection
Authors:	Ioana Lancrajan, Monica Horge, Doru Ardelean, Ioan Puscas
Affiliation:	„Vasile Goldiş” Western University of Arad, Faculty of Life Sciences, Romania Prof. Dr. Ioan Puscas” City Hospital Simleul-Silvaniei, Cosbuc Str. 29, Simleul Silvaniei, Romania
Abstract:	The ability of CagA, the most studied bacterial virulence factor of Helicobacter pylori to act in both phosphorylated and non phosphorylated form in order to activate multiple signal transduction pathways, promoting disruption of cell-cell contacts, migration and the typical hummingbird phenotype are under inverstigations. The wide variety of cagA effects, a different molecular forms phosphorylated/ nonphosphorylated, lead us to investigate the location of phosphorylation immediately after infection. The cytosolic (cyt) and non cytosolic (noncyt) fractions of host cells were investigated for the ability to phosphorylate cagA, in order to establish the location of components implied in phosphorylation. The results indicate that recombinant FL-cagA can not be phosphorylated only by cytosol or Non-cytosol from MDCK cells. The phosphorylation take place with phosphorylation reaction buffer (ATP, Mg+2, phosphatase inhibitors). That is an evidence for supporting hypothesis that specific kinases located on cell membranes are needed to perform phosphorylation.
Keywords:	infection, kinase, membrane, virulence factor, cagA, Helicobacter pylori
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*Correspondence:	Ioana Lancrajan, „Vasile Goldiş” Western University of Arad, Faculty of Life Sciences, Rebreanu str. 91-93; email: lancrajan _ioana@yahoo.com