Peroxidases activity in the germination time of the triticale caryopses after these were cryopreserved

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Article Title:	Peroxidases activity in the germination time of the triticale caryopses after these were cryopreserved
Authors:	Monica Şipoş, Dorina Cachiţă-Cosma, Alina Dora Samuel
Affiliation:	¹ Departament of Biology, University of Oradea, Romania ² Departament of Biology, “Vasile Goldiş” West University Arad, Romania
Abstract:	We followed the peroxidases activity (PA) – in first 44 hours of germination – in the exembryonated caryopses of triticale, after grain storage by immersion in liquid nitrogen (LN)(-196ºC) for variable periods of time: 5 minutes, 1 hour, 1 day, 1 week or 1 month. The cryopreservation of the triticale grain for 5 minutes or 1 hour has made that at 6 hours of germination the PA to be significantly decreased in relation with corresponding parameter in the control ex-embryonated caryopses and after 20 and 30 hours from placing the triticale caryopses to germinate decreases did not present statistic relevance; at 44 hours of germination has determined statistically insignificant stimulations of the PA. The cryopreservation of the triticale grains for 1 day in LN has determined – with relation to the control lot – a statistically insignificant inhibition of AP, after 6 and 20 hours of germination; further on, to 30 and 44 hours of germination there have been recorded stimulations of the activity of this enzyme, statistically relevant only at 30 hours from placing the caryopses to germinate. The cryopreservation of the triticale caryopses for 1 week or 1 month has made that at 6 hours of germination the PA activity to be insignificantly decreased in relation with corresponding parameter in the control grains. However, after 20, 30 and 44 hours from placing the triticale caryopses to germinate, stimulations of the PA have been registered; statistical relevance having the stimulations only at 30 hours of germination.
Keywords:	grains, Triticale, cryopreservation, liquid nitrogen (LN), peroxidases activity (PA)
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*Correspondence:	Şipoş M., University of Oradea, Department of Biology, no.1 Universităţii St., Oradea, Romania, email: siposmonica@yahoo.com