Ultrastructural features of Rhodophyta from the Black Sea Coast


Ultrastructural features of Rhodophyta from the Black Sea Coast

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Title: Ultrastructural features of Rhodophyta from the Black Sea Coast
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Article_Title: Ultrastructural features of Rhodophyta from the Black Sea Coast
Authors: Elena Doroftei, Manuela Rotaru-Stăncic, Liliana Miron
Affiliation: Faculty of Natural and Agricultural Science, “Ovidius” University, Constantza, Romania
Abstract: An important component of the oceanic and marine vegetation is represented by the algae, among which the macrophytes ones are an important link in the trophic net and an important biologic
resource. The present paper reports the results of our research of the morphology and ultrastructure of Polysiphonia denudata (Dillw.) And Ceramium rubrum (Huds). And completes a series of papers on the comparative ultrastructure of some Chlorophyta, Rhodophyta and Phaeophyta from the Romanian Black Sea Coast. Strips of thalli were cut out of the algal thallus from different regions: basal, middle and apical region. In light microscopy we used fresh thalli sectioned and observed at a Biorom photonic microscope, without colorants. Also, thin sections from algal thalli were processed by the electron microscopic technique through transmission and were observed under the performing electronic microscope Philips CM120. In our light microscope observations the branched filamentous habit is characteristic for both species studied. At the ultrastructural level the most distinctive features comparatively analyzed are: possession of simple plastids with unstacked thylakoids, two layered chloroplast envelope, lack of chloroplasts ER, pit-connection or pit plug, floridean starch as the storage product, which lies freely in the cytoplasm, the thallus heaving unicleate cells, the nucleus being small and with one or two nucleoli and cell wall composed of cellulose with the fibrils randomly arranged.
Keywords: algae, Polysiphonia denudata, Ceramium rubrum, thallus, chloroplast, thylakoid, floridean starch,
pit plug
References: Alley CD, Scott JL, Unusual dictyosome morphology and vesicle formation in tetrasporangia of the
marine red alga Polysiphonia denudata. J. Ultrastr. Res. 58, 289-298, 1977.
Athanasiadis A, Morphology and classification of the Ceramioidae (Rhodophyta) based on phylogenetic principles. Opera Botanica, 128, 1, 216, 1996.
Bavaru A, Bologa AS, Skolka VH, A checklist of the benthic marine algae (except diatoms) along the
Romanian Shore of the Black Sea – Oebalia. Int. Jour. Mar. Biol. Ocean., 17, 2 (suppl.), Toronto, Italia, 535-551, 1991.
Bavaru A, Brezeanu A, Doroftei E, Jianu L, Ultrastructure of algal cells at Ceramium rubrum (Huds) C. Ag. In: Current problems in cellular and molecular biology, IV, 562-566, 1999. Edited by Ardelean A. şi Crăciun C., Ed. Risoprint, Cluj-Napoca.
Bavaru A, Brezeanu A, Doroftei E, Jianu L, Ultrastructural aspects at multicellular green
seaweeds Ulva rigida Ag., Enteromorpha intestinalis (L.) Link, Cladophora sericea (Huds.) Kutz. In: Current problems in cellular and molecular biology, V, 567-575, 2000. Edited by A. Ardelean şi C. Crăciun. Ed. Risoprint, Cluj-Napoca.
Bold HC, Wynne MJ, Introduction to the Algae. Structure and reproduction, 706, 1978. Ed. University Press, Cambridge.
Boo SM, Intermediately corticated species, Ceramium puberubem (Ceramiaceae, Rhodophyta). Jpn. J.
Phycol (sorui) 41, 143-149, 1993.
Boo SM, Lee AK, Ceramium and Campylaephora (Ceramiaceae, Rhodophyta). In: Biology of Economic Algae, 1-33, 1994. Edited by I. Akatsuka. SPB Academic Publishing, The Hague.
Cho TO, Riosmena-Rodríguez R, Boo SM, First record of Ceramium giacconei (Ceramiaceae, Rhodophyta) in the North Pacific: developmental morphology of vegetative and reproductive structures. Botanica Marina 46, 548-554, 2003.
Doroftei E, Bavaru A, Brezeanu A, Sava D, Comparative ultrastructure of some Chlorophyta and Rhodophyta from the Romanian coast of the Black Sea. In: Current problems in cellular and molecular biology, V, 462 – 470, 2000. Edited by Crăciun C. şi Ardelean A. Ed. Risoprint, Cluj-Napoca
Doroftei E, Sava D, Brezeanu A, Moldoveanu D, Particularităţi citofiziologice la Ulva rigida şi
Cladophora vagabunda în condiţiile cultivării “in vitro”. In: Probleme curente de biologie
celulară si moleculară, VII, 476-483, 2002. Edited by C. Crăciun şi A. Ardelean. Ed. Risoprint, Cluj-Napoca.
Doroftei E, Sava D, Brezeanu A, Arcuş M, Modificãri ultrastructurale la algele marine verzi
pluricelulare în condiţiile cultivãrii „in vitro”, VIII, 202-212, 2003. In: Analele Societãţii
Naţionale de Biologie Celularã. Edited by C. Crăciun şi A. Ardelean. Ed. Risoprint, Cluj-Napoca.
Doroftei E, Sava D, Mihăilă F, Aspecte ultrastructurale ale celulei algale la Ceramium elegans Ducl. In: Analele Societãţii Naţionale de Biologie Celularã, IX, 1, 357-370, 2004. Edited by C.
Crăciun şi A. Ardelean. Ed. Risoprint, Cluj-Napoca.
Doroftei E, Sava D, Bavaru A, Modificãri ultrastructurale la algele marine verzi pluricelulare, sub acţiunea unor metale grele. In: Analele Societãţii Naţionale de Biologie Celularã, X, 467-473, 2005. Edited by C. Crăciun şi A. Ardelean. Ed. Risoprint, Cluj-Napoca.
Doroftei E, Sava D, Bavaru A, Zamfirescu S, Ultrastructura celulei algale la Bryopsis plumosa (Huds) Ag. In: Analele Societãţii Naţionale de Biologie Celularã, XI, 554-562, 2006a. Edited by C. Crăciun şi A. Ardelean. Ed. Risoprint, Cluj-Napoca.
Doroftei E, Jianu L, Brezeanu A, Bavaru A, Particularitati citologice la Cystoseira barbata (Good et. Wood Ag.). In: Analele Societãţii Naţionale de Biologie Celularã, XI, 563-571, 2006b. Edited by C. Crăciun şi A. Ardelean. Ed. Risoprint, Cluj-Napoca.
Edwards P, The life history of Polysiphonia denudata (Dillwyn) Kützing in culture. Journal of
Phycology 4, 35-37, 1968.
Edwards P, Field and cultural observations on the growth and reproduction of Polysiphonia denudata from Texas. British Phycological Journal 5, 145-153, 1970.
Fujii MT, Concentino ALM, Pereira, SMB, Ceramium nitens (Ceramiaceae, Rhodophyta), an uncommon species from Brazil. Rev. Bras. Bot., 24, 3, 359-363, 2001.
Hayat MA, Basic Electron Microscopy Techniques, 2, 1972. Ed. Van Nostrand Reinhold Company. Itono H, The genus Ceramium (Ceramiaceae, Rhodophyta) in southern Japan. Botanica Marina, 15, 74-86, 1972.
Reynolds E S, The use of lead citrate at high pH as an electron-opaque stain in electronmicroscopy. J. Cell Biol., 17, 208, 1963.
Van den Hoeck, Algae: an introduction to phycology, 300-342; 419-436, 1997. Ed. Cambridge University Press, Cambridge. Womersley HBS, Southern Australian species of Ceramium Roth Greville (Rhodophyta). Australian Journal of Marine and Freshwater Research, 29, 205-257, 1978.
Read_full_article: pdf/18-2008/SU08Doroftei.pdf
Correspondence: Elena Doroftei, Faculty of Natural and Agricultural Science, “Ovidius” University, Constantza, no.
124 Mamaia Blv., Constantza, 900527, Romania, Tel. +40-(241)-614576-interior 39, email: edoroftei2000@yahoo.ca

Read full article
Article Title: Ultrastructural features of Rhodophyta from the Black Sea Coast
Authors: Elena Doroftei, Manuela Rotaru-Stăncic, Liliana Miron
Affiliation: Faculty of Natural and Agricultural Science, “Ovidius” University, Constantza, Romania
Abstract: An important component of the oceanic and marine vegetation is represented by the algae, among which the macrophytes ones are an important link in the trophic net and an important biologic
resource. The present paper reports the results of our research of the morphology and ultrastructure of Polysiphonia denudata (Dillw.) And Ceramium rubrum (Huds). And completes a series of papers on the comparative ultrastructure of some Chlorophyta, Rhodophyta and Phaeophyta from the Romanian Black Sea Coast. Strips of thalli were cut out of the algal thallus from different regions: basal, middle and apical region. In light microscopy we used fresh thalli sectioned and observed at a Biorom photonic microscope, without colorants. Also, thin sections from algal thalli were processed by the electron microscopic technique through transmission and were observed under the performing electronic microscope Philips CM120. In our light microscope observations the branched filamentous habit is characteristic for both species studied. At the ultrastructural level the most distinctive features comparatively analyzed are: possession of simple plastids with unstacked thylakoids, two layered chloroplast envelope, lack of chloroplasts ER, pit-connection or pit plug, floridean starch as the storage product, which lies freely in the cytoplasm, the thallus heaving unicleate cells, the nucleus being small and with one or two nucleoli and cell wall composed of cellulose with the fibrils randomly arranged.
Keywords: algae, Polysiphonia denudata, Ceramium rubrum, thallus, chloroplast, thylakoid, floridean starch,
pit plug
References: Alley CD, Scott JL, Unusual dictyosome morphology and vesicle formation in tetrasporangia of the
marine red alga Polysiphonia denudata. J. Ultrastr. Res. 58, 289-298, 1977.
Athanasiadis A, Morphology and classification of the Ceramioidae (Rhodophyta) based on phylogenetic principles. Opera Botanica, 128, 1, 216, 1996.
Bavaru A, Bologa AS, Skolka VH, A checklist of the benthic marine algae (except diatoms) along the
Romanian Shore of the Black Sea – Oebalia. Int. Jour. Mar. Biol. Ocean., 17, 2 (suppl.), Toronto, Italia, 535-551, 1991.
Bavaru A, Brezeanu A, Doroftei E, Jianu L, Ultrastructure of algal cells at Ceramium rubrum (Huds) C. Ag. In: Current problems in cellular and molecular biology, IV, 562-566, 1999. Edited by Ardelean A. şi Crăciun C., Ed. Risoprint, Cluj-Napoca.
Bavaru A, Brezeanu A, Doroftei E, Jianu L, Ultrastructural aspects at multicellular green
seaweeds Ulva rigida Ag., Enteromorpha intestinalis (L.) Link, Cladophora sericea (Huds.) Kutz. In: Current problems in cellular and molecular biology, V, 567-575, 2000. Edited by A. Ardelean şi C. Crăciun. Ed. Risoprint, Cluj-Napoca.
Bold HC, Wynne MJ, Introduction to the Algae. Structure and reproduction, 706, 1978. Ed. University Press, Cambridge.
Boo SM, Intermediately corticated species, Ceramium puberubem (Ceramiaceae, Rhodophyta). Jpn. J.
Phycol (sorui) 41, 143-149, 1993.
Boo SM, Lee AK, Ceramium and Campylaephora (Ceramiaceae, Rhodophyta). In: Biology of Economic Algae, 1-33, 1994. Edited by I. Akatsuka. SPB Academic Publishing, The Hague.
Cho TO, Riosmena-Rodríguez R, Boo SM, First record of Ceramium giacconei (Ceramiaceae, Rhodophyta) in the North Pacific: developmental morphology of vegetative and reproductive structures. Botanica Marina 46, 548-554, 2003.
Doroftei E, Bavaru A, Brezeanu A, Sava D, Comparative ultrastructure of some Chlorophyta and Rhodophyta from the Romanian coast of the Black Sea. In: Current problems in cellular and molecular biology, V, 462 – 470, 2000. Edited by Crăciun C. şi Ardelean A. Ed. Risoprint, Cluj-Napoca
Doroftei E, Sava D, Brezeanu A, Moldoveanu D, Particularităţi citofiziologice la Ulva rigida şi
Cladophora vagabunda în condiţiile cultivării “in vitro”. In: Probleme curente de biologie
celulară si moleculară, VII, 476-483, 2002. Edited by C. Crăciun şi A. Ardelean. Ed. Risoprint, Cluj-Napoca.
Doroftei E, Sava D, Brezeanu A, Arcuş M, Modificãri ultrastructurale la algele marine verzi
pluricelulare în condiţiile cultivãrii „in vitro”, VIII, 202-212, 2003. In: Analele Societãţii
Naţionale de Biologie Celularã. Edited by C. Crăciun şi A. Ardelean. Ed. Risoprint, Cluj-Napoca.
Doroftei E, Sava D, Mihăilă F, Aspecte ultrastructurale ale celulei algale la Ceramium elegans Ducl. In: Analele Societãţii Naţionale de Biologie Celularã, IX, 1, 357-370, 2004. Edited by C.
Crăciun şi A. Ardelean. Ed. Risoprint, Cluj-Napoca.
Doroftei E, Sava D, Bavaru A, Modificãri ultrastructurale la algele marine verzi pluricelulare, sub acţiunea unor metale grele. In: Analele Societãţii Naţionale de Biologie Celularã, X, 467-473, 2005. Edited by C. Crăciun şi A. Ardelean. Ed. Risoprint, Cluj-Napoca.
Doroftei E, Sava D, Bavaru A, Zamfirescu S, Ultrastructura celulei algale la Bryopsis plumosa (Huds) Ag. In: Analele Societãţii Naţionale de Biologie Celularã, XI, 554-562, 2006a. Edited by C. Crăciun şi A. Ardelean. Ed. Risoprint, Cluj-Napoca.
Doroftei E, Jianu L, Brezeanu A, Bavaru A, Particularitati citologice la Cystoseira barbata (Good et. Wood Ag.). In: Analele Societãţii Naţionale de Biologie Celularã, XI, 563-571, 2006b. Edited by C. Crăciun şi A. Ardelean. Ed. Risoprint, Cluj-Napoca.
Edwards P, The life history of Polysiphonia denudata (Dillwyn) Kützing in culture. Journal of
Phycology 4, 35-37, 1968.
Edwards P, Field and cultural observations on the growth and reproduction of Polysiphonia denudata from Texas. British Phycological Journal 5, 145-153, 1970.
Fujii MT, Concentino ALM, Pereira, SMB, Ceramium nitens (Ceramiaceae, Rhodophyta), an uncommon species from Brazil. Rev. Bras. Bot., 24, 3, 359-363, 2001.
Hayat MA, Basic Electron Microscopy Techniques, 2, 1972. Ed. Van Nostrand Reinhold Company. Itono H, The genus Ceramium (Ceramiaceae, Rhodophyta) in southern Japan. Botanica Marina, 15, 74-86, 1972.
Reynolds E S, The use of lead citrate at high pH as an electron-opaque stain in electronmicroscopy. J. Cell Biol., 17, 208, 1963.
Van den Hoeck, Algae: an introduction to phycology, 300-342; 419-436, 1997. Ed. Cambridge University Press, Cambridge. Womersley HBS, Southern Australian species of Ceramium Roth Greville (Rhodophyta). Australian Journal of Marine and Freshwater Research, 29, 205-257, 1978.
*Correspondence: Elena Doroftei, Faculty of Natural and Agricultural Science, “Ovidius” University, Constantza, no.
124 Mamaia Blv., Constantza, 900527, Romania, Tel. +40-(241)-614576-interior 39, email: edoroftei2000@yahoo.ca