Effects of Juniperus communis aerosols on trachea and lung from rat subjected to passive smoking

Effects of Juniperus communis aerosols on trachea and lung from rat subjected to passive smoking

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Title: Effects of Juniperus communis aerosols on trachea and lung from rat subjected to passive smoking
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Article_Title: Effects of Juniperus communis aerosols on trachea and lung from rat subjected to passive smoking
Authors: Carmen-Manuela Pleşa, Valentin Laurenţiu Ordodi, Lavinia Noveanu, Liliana Vasile, Aurel Ardelean, Alfa Xenia Lupea
Affiliation: 1 Faculty of Natural Sciences and Environmental Protection, “Vasile Goldiş” Western University of Arad, Arad, Romania
2 Faculty of Industrial Chemistry and Environmental Engineering,
“Politehnica” University of Timişoara, Timişoara, Romania
3 University of Medicine and Pharmacy “Victor Babeş”, Timişoara, Romania
Abstract: The effect of cigarette smoking and essential oil of Juniperus communis of tracheal contraction and dilatation was studied in 40 female Spraque-Dawley rats. The animals (rats) that were used for analysis were divided into four lots: smoking, non-smoking, exposed and not exposed to juniper oil. The smoking lot was exposed to 2 cigarettes per day, 5 days a week for 6 weeks. Then, the rats belonging to lots 2 and 4 were exposed to juniper berries oil for 3 weeks, 20 minutes per day. The control lot (lot 1) was not exposed to cigarette smoke. In this study we also performed physiological analyses for trachea and lung of experimental rats. Histopathological studies present the effect of cigarette smoke and Juniperus communis oil for trachea and lung. Physiological studies were performed upon analysis of bronhodilator and bronhoconstrictor trachea effects.
Keywords: juniper, rats, smoke, trachea, lung
References: Atoji Y, Kusindarta DL, Hamazaki N, Kaneko A (2005) Innervation of the rat trachea by bilateral
cholinergic projections from the nucleus ambiguus and direct motor fibers from the cervical spinal
cord: a retrograde and anterograde tracer study. Brain Research, 1031(7), pp. 90-100.
Barnes PJ (2001) Neurogenic inflammation in the airways. Respiration Physiology, 125 (1-2), pp. 145-
154.
Butkienė R, Nivinskienė O, Mockutė D, (2009) Two chemotypes of essential oils produced by the same
Juniperus communis L. growing wild in Lithuania. Chemija, 20, (3), pp. 196–202.
Cavaleiro C, Salgueiro LR, Figueiredo AC, Barroso JG, Bighelli A, Casanova J, (2003) Composition and
variability of the essential oils of the leaves and berries from Juniperus navicularis. Biochemical
Systematics and Ecology, 31, pp. 193–201.
Kobzik L, Bredt DS, Lowenstein CJ, Drazen J, Gaston B, Sugarbaker D, Stamler JS (1993) Nitric oxide
synthase in human and rat lung: immunocytochemical and histochemical localization. Am J Respir Cell
Mol Biol., 9(4), pp. 371-377.
Kuo PC, Schroeder RA (1995) The Emerging Multifaceted Roles of Nitric Oxide. Annals of Surgery, 221(3), pp. 220-235.
Kusindarta DL, Atoji Y, Yamamoto Y (2004) Nerve plexuses in the trachea and extrapulmonary bronchi
of the rat. Archives of Histology and Cytology, 67 (1), pp. 41-55
Maria GM (2010) Antioxidant and anti-inflammatory activities of Essential Oils: a short review.
Molecules, 15, pp. 9252-9287.
Mihalas G, Bunu C, Noveanu L, Siska IR, Borza C, Tănasie G (2003) Responsivitatea bronşică. De
la experiment la studiu clinic,. Editura Orizonturi Universitare, Timişoara, ISBN 973 – 638 – 84 – X.
Munteanu I, Didilescu C (2007) Chimia şi toxicologia fumului de tigara la nivel pulmonar. Pneumologia, 56 (1), pp. 41-46.
Ricciardolo FLM (2005) Multiple roles of nitric oxide in the airways. Thorax, 58, pp. 175-182.
Sekizawa K, Fukushima T, Ikarashi Y, Maruyama Y, Sasaki H (1993) The role of nitric oxide in
cholinergic neurotransmission in rat trachea. Br J Pharmacol., 110(2), pp. 816–820.
Sezik E, Kocakulak E, Baser KHC, Ozek T, (2005) Composition of the Essential Oils of Juniperus
oxycedrus subsp. macrocarpa from Turkey. Chemistry of Natural Compounds, 41, No. 3, pp. 352-354.
Shahmir F, Ahmadi L, Mirza M, Korori SAA, (2003) Secretory elements of needles and berries of
Juniperus communis L. ssp. communis and its volatile constituents. Flavour Fragr. J., 18, pp. 425–
428, DOI: 10.1002/ffj.1243.
Tunalier Z, Kirimer N, Baser KHC, (2002) The Composition of Essential Oils from Various Parts of Juniperus foetidissima. Chemistry of Natural Compounds, 38(1), pp. 43-47.
Read_full_article: pdf/21-2011/21-2-2011/SU21-2-2011Plesa.pdf
Correspondence: e-mail: manuelita_carmen@yahoo.com

Read full article
Article Title: Effects of Juniperus communis aerosols on trachea and lung from rat subjected to passive smoking
Authors: Carmen-Manuela Pleşa, Valentin Laurenţiu Ordodi, Lavinia Noveanu, Liliana Vasile, Aurel Ardelean, Alfa Xenia Lupea
Affiliation: 1 Faculty of Natural Sciences and Environmental Protection, “Vasile Goldiş” Western University of Arad, Arad, Romania
2 Faculty of Industrial Chemistry and Environmental Engineering,
“Politehnica” University of Timişoara, Timişoara, Romania
3 University of Medicine and Pharmacy “Victor Babeş”, Timişoara, Romania
Abstract: The effect of cigarette smoking and essential oil of Juniperus communis of tracheal contraction and dilatation was studied in 40 female Spraque-Dawley rats. The animals (rats) that were used for analysis were divided into four lots: smoking, non-smoking, exposed and not exposed to juniper oil. The smoking lot was exposed to 2 cigarettes per day, 5 days a week for 6 weeks. Then, the rats belonging to lots 2 and 4 were exposed to juniper berries oil for 3 weeks, 20 minutes per day. The control lot (lot 1) was not exposed to cigarette smoke. In this study we also performed physiological analyses for trachea and lung of experimental rats. Histopathological studies present the effect of cigarette smoke and Juniperus communis oil for trachea and lung. Physiological studies were performed upon analysis of bronhodilator and bronhoconstrictor trachea effects.
Keywords: juniper, rats, smoke, trachea, lung
References: Atoji Y, Kusindarta DL, Hamazaki N, Kaneko A (2005) Innervation of the rat trachea by bilateral
cholinergic projections from the nucleus ambiguus and direct motor fibers from the cervical spinal
cord: a retrograde and anterograde tracer study. Brain Research, 1031(7), pp. 90-100.
Barnes PJ (2001) Neurogenic inflammation in the airways. Respiration Physiology, 125 (1-2), pp. 145-
154.
Butkienė R, Nivinskienė O, Mockutė D, (2009) Two chemotypes of essential oils produced by the same
Juniperus communis L. growing wild in Lithuania. Chemija, 20, (3), pp. 196–202.
Cavaleiro C, Salgueiro LR, Figueiredo AC, Barroso JG, Bighelli A, Casanova J, (2003) Composition and
variability of the essential oils of the leaves and berries from Juniperus navicularis. Biochemical
Systematics and Ecology, 31, pp. 193–201.
Kobzik L, Bredt DS, Lowenstein CJ, Drazen J, Gaston B, Sugarbaker D, Stamler JS (1993) Nitric oxide
synthase in human and rat lung: immunocytochemical and histochemical localization. Am J Respir Cell
Mol Biol., 9(4), pp. 371-377.
Kuo PC, Schroeder RA (1995) The Emerging Multifaceted Roles of Nitric Oxide. Annals of Surgery, 221(3), pp. 220-235.
Kusindarta DL, Atoji Y, Yamamoto Y (2004) Nerve plexuses in the trachea and extrapulmonary bronchi
of the rat. Archives of Histology and Cytology, 67 (1), pp. 41-55
Maria GM (2010) Antioxidant and anti-inflammatory activities of Essential Oils: a short review.
Molecules, 15, pp. 9252-9287.
Mihalas G, Bunu C, Noveanu L, Siska IR, Borza C, Tănasie G (2003) Responsivitatea bronşică. De
la experiment la studiu clinic,. Editura Orizonturi Universitare, Timişoara, ISBN 973 – 638 – 84 – X.
Munteanu I, Didilescu C (2007) Chimia şi toxicologia fumului de tigara la nivel pulmonar. Pneumologia, 56 (1), pp. 41-46.
Ricciardolo FLM (2005) Multiple roles of nitric oxide in the airways. Thorax, 58, pp. 175-182.
Sekizawa K, Fukushima T, Ikarashi Y, Maruyama Y, Sasaki H (1993) The role of nitric oxide in
cholinergic neurotransmission in rat trachea. Br J Pharmacol., 110(2), pp. 816–820.
Sezik E, Kocakulak E, Baser KHC, Ozek T, (2005) Composition of the Essential Oils of Juniperus
oxycedrus subsp. macrocarpa from Turkey. Chemistry of Natural Compounds, 41, No. 3, pp. 352-354.
Shahmir F, Ahmadi L, Mirza M, Korori SAA, (2003) Secretory elements of needles and berries of
Juniperus communis L. ssp. communis and its volatile constituents. Flavour Fragr. J., 18, pp. 425–
428, DOI: 10.1002/ffj.1243.
Tunalier Z, Kirimer N, Baser KHC, (2002) The Composition of Essential Oils from Various Parts of Juniperus foetidissima. Chemistry of Natural Compounds, 38(1), pp. 43-47.
*Correspondence: e-mail: manuelita_carmen@yahoo.com