Stability of effector protein cagA: a prerequisite for HP physiology and pathology


Stability of effector protein cagA: a prerequisite for HP physiology and pathology

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Title: Stability of effector protein cagA: a prerequisite for HP physiology and pathology
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Article_Title: Stability of effector protein cagA: a prerequisite for HP physiology and pathology
Authors: Ioana Lancrajan
Affiliation: „Vasile Goldiş” Western University of Arad, Faculty of Life Sciences, Romania
Abstract: Helicobacter pylori colonizes the human stomach and can induce gastritis, peptic ulceration, gastric MALT lymphoma and gastric adenocarcinoma, the second most common cause of cancer mortality in the world. We suppose that the role of effector protein CagA in pathology of HP infection is partially influenced by a very diverse and extreme environment in the stomach, with consequences on the structure and functionality of CagA and hence on physiopatology of HP. The purpose of this study is to evaluate structural and functional CagA, the single known effector protein of Helicobacter pylori under physiological conditions.Our result indicate CagA as a very susceptible molecule to degradation dependent on medium factors. Recombinant CagA shown a very high instability and degradation under different physical-chemical factors, selective degradation especially at the functional significantly C-terminus, high sensitivity and degradation by mechanical stress and high temperature, higher temperature favouring faster degradation as lower temperature.
Keywords: stability, effector protein, environment, degradation, stomach
References: Arakawa, T., Philo, J. S., Ejima, D., Tsumoto, K., and Arisaka, F., 2006, Aggregation analysis of
therapeutic proteins, part 1: General aspects and techniques for assessment, Bioprocess International 4 (10), 42-49.
Bourzac, K. M., and Guillemin, K., 2005, Helicobacter pylori- host cell interactions mediated by type IV secretion. Cellular Microbiology, 7, 911-919.
Covacci, A., Censini, S., Bugnoli, M., Petracca, R., Burroni, D., Macchia, G., Massone, A., Papini,
E., Xiang, Z., Figura, N., and Rappouli, R., 1993, Molecular characterization of the 128-kDa
immunodominant antigen of Helicobacter pylori associated with cytotoxicity and duodenal ulcer.,
PNAS, 90, 5791-5795
Cromwell MEM, 2007, Biotechnology derived drug products: formulation development. In: Encyclopedia of Pharmaceutical Technology, J. Swarbrick Ed., Informa Healthcare UISA 282-301
Gauthier, N. C., Monzo, P., Gonzalez, T., Doye, A., Oldani, A., Gounon, P., Ricci, V., Cormont, M., and Boquet, P., 2007, Early endosomes associated with dynamic F-actin structures are required for late trafficking of H. pylori VacA toxin, J. Cell Biol. 177, 343–354.
Hisatsune, J., Nakayama, M., Isomoto, H., Kurazono, H., Mukaida, N., Mukhopadhyay, A. K., Azuma, T.,
Yamaoka, Y., Sap, J., Yamasaki, E., Yahiro, K., Moss, J., and Hirayama, T., 2008, Molecular characterization of Helicobacter pylori VacA induction of IL-8 in U937 cells reveals a prominent
role for p38MAPK in activating transcription factor-2, cAMP response element binding protein, and NF-kappaB activation, J. Immunol. 180, 5017–5027.
Tegtmeyer N., Backert S., 2010, Role of Abl and Src family kinases in actin-cytoskeletal rearrangements induced by the Helicobacter pylori CagA protein, European Journal of Cell Biology, in press
Yamasaki, E., Wada, A., Kumatori, A., Nakagawa, I., Funao, J., Nakayama, M., Hisatsune, J., Kimura,
M., Moss, J., and Hirayama, T., 2006, Helicobacter pylori vacuolating cytotoxin induces activation of the proapoptotic proteins Bax and Bak, leading to cytochrome c release and cell death, independent of vacuolation, J. Biol. Chem. 281, 11250–11259.
Read_full_article: pdf/21-2011/21-2-2011/SU21-2-2011Lancrajan.pdf
Correspondence: Ioana Lancrajan, „Vasile Goldiş” Western University of Arad, Faculty of Life Sciences,
Email: Lancrajan _ioana@yahoo.com

Read full article
Article Title: Stability of effector protein cagA: a prerequisite for HP physiology and pathology
Authors: Ioana Lancrajan
Affiliation: „Vasile Goldiş” Western University of Arad, Faculty of Life Sciences, Romania
Abstract: Helicobacter pylori colonizes the human stomach and can induce gastritis, peptic ulceration, gastric MALT lymphoma and gastric adenocarcinoma, the second most common cause of cancer mortality in the world. We suppose that the role of effector protein CagA in pathology of HP infection is partially influenced by a very diverse and extreme environment in the stomach, with consequences on the structure and functionality of CagA and hence on physiopatology of HP. The purpose of this study is to evaluate structural and functional CagA, the single known effector protein of Helicobacter pylori under physiological conditions.Our result indicate CagA as a very susceptible molecule to degradation dependent on medium factors. Recombinant CagA shown a very high instability and degradation under different physical-chemical factors, selective degradation especially at the functional significantly C-terminus, high sensitivity and degradation by mechanical stress and high temperature, higher temperature favouring faster degradation as lower temperature.
Keywords: stability, effector protein, environment, degradation, stomach
References: Arakawa, T., Philo, J. S., Ejima, D., Tsumoto, K., and Arisaka, F., 2006, Aggregation analysis of
therapeutic proteins, part 1: General aspects and techniques for assessment, Bioprocess International 4 (10), 42-49.
Bourzac, K. M., and Guillemin, K., 2005, Helicobacter pylori- host cell interactions mediated by type IV secretion. Cellular Microbiology, 7, 911-919.
Covacci, A., Censini, S., Bugnoli, M., Petracca, R., Burroni, D., Macchia, G., Massone, A., Papini,
E., Xiang, Z., Figura, N., and Rappouli, R., 1993, Molecular characterization of the 128-kDa
immunodominant antigen of Helicobacter pylori associated with cytotoxicity and duodenal ulcer.,
PNAS, 90, 5791-5795
Cromwell MEM, 2007, Biotechnology derived drug products: formulation development. In: Encyclopedia of Pharmaceutical Technology, J. Swarbrick Ed., Informa Healthcare UISA 282-301
Gauthier, N. C., Monzo, P., Gonzalez, T., Doye, A., Oldani, A., Gounon, P., Ricci, V., Cormont, M., and Boquet, P., 2007, Early endosomes associated with dynamic F-actin structures are required for late trafficking of H. pylori VacA toxin, J. Cell Biol. 177, 343–354.
Hisatsune, J., Nakayama, M., Isomoto, H., Kurazono, H., Mukaida, N., Mukhopadhyay, A. K., Azuma, T.,
Yamaoka, Y., Sap, J., Yamasaki, E., Yahiro, K., Moss, J., and Hirayama, T., 2008, Molecular characterization of Helicobacter pylori VacA induction of IL-8 in U937 cells reveals a prominent
role for p38MAPK in activating transcription factor-2, cAMP response element binding protein, and NF-kappaB activation, J. Immunol. 180, 5017–5027.
Tegtmeyer N., Backert S., 2010, Role of Abl and Src family kinases in actin-cytoskeletal rearrangements induced by the Helicobacter pylori CagA protein, European Journal of Cell Biology, in press
Yamasaki, E., Wada, A., Kumatori, A., Nakagawa, I., Funao, J., Nakayama, M., Hisatsune, J., Kimura,
M., Moss, J., and Hirayama, T., 2006, Helicobacter pylori vacuolating cytotoxin induces activation of the proapoptotic proteins Bax and Bak, leading to cytochrome c release and cell death, independent of vacuolation, J. Biol. Chem. 281, 11250–11259.
*Correspondence: Ioana Lancrajan, „Vasile Goldiş” Western University of Arad, Faculty of Life Sciences,
Email: Lancrajan _ioana@yahoo.com