Phase solubility studies and scanning electron microscopy of dexamethasone inclusion complexes with B-cyclodextrin and hydroxypropyl B-cyclodextrin

Phase solubility studies and scanning electron microscopy of dexamethasone inclusion complexes with B-cyclodextrin and hydroxypropyl B-cyclodextrin

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Title: Phase solubility studies and scanning electron microscopy of dexamethasone inclusion complexes with B-cyclodextrin and hydroxypropyl B-cyclodextrin
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Article_Title: Phase solubility studies and scanning electron microscopy of dexamethasone inclusion complexes with B-cyclodextrin and hydroxypropyl B-cyclodextrin
Authors: Mariuca-Roxana Gavriloaia, Emma-Adriana Budura, Corina Arama, Mihai Nitulescu, Dumitru Lupuleasa
Affiliation: Pharmaceutical Technology Department, Analytical Chemistry Department
„Carol Davila” University of Medicine and Pharmacy, Bucharest,
Abstract: Dexamethasone, Dxm, is frequently used as an immunosuppressive to treat a broad range of autoimmune and inflammatory disorders, most of them occur locally and near the body surface. So, topical application could offer the advantage of delivering a drug directly to the affected surface, but barrier properties of skin limit the dexamethasone’s permeability. Transdermal penetration could be improved by drug complexation with cyclodextrins. The purpose of this paper is to analyze the formation of inclusion complexes of the drug with β-cyclodextrin and hydroxypropyl-β-cyclodextrin. Investigations were made by phase solubility diagrams and scanning electron microscopy in order to evaluate: the formation of 1:1 molar ratios, complexation efficiency, improved solubility, morphology and homogeneity samples. Huguchi and Connors method and fractal analysis were applied to compare the performances of the two final binary solutions.
Keywords: dexamethasone, cyclodextrins, inclusion complex, phase solubility diagram, fractal analysis
References: Aleem O., Kuchekar B., Pore Y., Late S., Effect of β- cyclodextrin and hydroxypropyl-β-cyclodextrin complexation on physicochemical properties and antimicrobial activity of cefdinir, J.Pharm.Biomed. Anal, 47, 535-540, 2008,
Armatas G.S., Kolonia K.M., and Pomonis P.J., Morphometry of porous solids: lacunarity, fractal dimensions, connectivity, and some topological similarities with neurons. Langmuir, 18, 26, 3552-3558, 2002,
Bibby D. C., Davies N . M., and Tucker I. G., Mechanisms by which cyclodextrins modify drug release from polymeric drug delivery systems. Int. J. Pharm. 197:1–11, 2000,
Brewster M.E., Loftsson T., Cyclodextrins as pharmaceutical solubilizers, Adv.Drug Deliv.Rev., 59, 645-666, 2007,
Higuchi T., Connors K.A., Phase-solubility techniques, Adv.Anal.Chem.Instr., 4, 117-122, 1965, Cázares D. J., Fernández C.B., Pascual A. C., Rondero A., G., Guerrero D.Q., Castellano L., Merino V., Kalia V., Transdermal iontophoresis of dexamethasone sodium phosphate in vitro and in vivo: Effect of experimental parameters and skin type on drug stability and transport kinetics, European Journal of Pharmaceutics and Biopharmaceutics Volume 75, Issue 2, June, p 173-178, 2010, Davies E.R., Handbook of Texture Analysis, Chapter 1— Introduction to texture analysis, Imperial College Press, London, UK, 2008,
Dong P., Lacunarity analysis of raster datasets and 1d, 2d, and 3d point patterns. Computers and Geosciences, 35, 2009,
Fansook P., Ritthidej G., Ueda H., Stefánsson E., Loftsson T., γCD/HPγCD Mixtures as Solubilizer: Solid- State Characterization and Sample Dexamethasone Eye Drop Suspension, J Pharm Pharmaceut Sci
(www.cspsCanada.org) 13(3), p 336-350, 2010,
Faula D., Oliveira D., Tedesco A.C., Bentley M. V., Enhancing effect of modified beta-cyclodextrins on in vitro skin permeation of estradiol, Revista Brasileira de Ciências Farmacêuticas, Brazilian Journal of Pharmaceutical Sciences, vol. 43, n. 1, jan./mar., 2007,
Gavriloaia M.R, Creţu E.A, Lupuleasa D, Infrared studies on the thermal efect of topical glucocorticoids/ cyclodextrins complexes, ACTA MEDICA MARISIENSIS, Volume 56, Supplement 2, The XIVth National Congress of Pharmacy from Romania, Tîrgu-Mureş, October 13th – 16th, 2010,
Official publication of the University of Medicine and Pharmacy of Târgu Mureş, ISSN 2068-3324, pag. 100, 2010.
Hagiwara T., Kumagai H., and Nakamura K., Fractal analysis of aggregates in heat-induced BSA gels. Food Hydrocolloids, 12, 1, p 1756-1763, 1998,
Karperien A., FracLac advanced user’s manual, Charles Sturt University, Australia, 2004,Lee T., Atkins,M. S., A new approach to measure border irregularity for melanocytic lesions, Medical Imaging 2000: Image Processing, Proceedings of SPIE Vol. 3979, p 250-256, 2000,
Luo L., and Lin H., Lacunarity and fractal analyses of soil macropores and preferential transport using micro-X-ray computed tomography. Vadose Zone Journal, 8, p 233-241, 2009,
Ozkan Y., Atay T., Dikmen N., Işimer A., Aboul-Enein H.Y., Improvement of water solubility and in vitro dissolution rate of gliclazide by complexation with β –cyclodextrin, Pharm. Acta Helv., 74, Apr;74(4):365-70. 2000,
Sonka M., Hlavac V., Boyle R., Texture, in: Image Processing, Analysis, and Machine Vision, third ed., Thomson Learning, Ontario, Canada, 2008,
Stella V. J., Rajewski R. A., Cyclodextrins: Their future in drug formulation and delivery, Pharm. Res., May; 14(5): 556-5671997:
Vianna R. F. L., Bentley M. V., Ribeiro L. B. G et al. Formation of cyclodextrin inclusion complexes with corticosteroids: their characterization and stability. Int. J. Pharm. V. 167, p.205-213, 1998.
Read_full_article: pdf/22-2012/22-1-2012/SU22-1-2012-Gavriloaia.pdf
Correspondence: Mariuca-Roxana GAVRILOAIA*, „Carol Davila” University of Medicine and Pharmacy, Bucharest, Faculty of Pharmacy, 6 Traian Vuia St., 020956, Bucharest, Romania; e-mail: roxana21g@yahoo.com.

Read full article
Article Title: Phase solubility studies and scanning electron microscopy of dexamethasone inclusion complexes with B-cyclodextrin and hydroxypropyl B-cyclodextrin
Authors: Mariuca-Roxana Gavriloaia, Emma-Adriana Budura, Corina Arama, Mihai Nitulescu, Dumitru Lupuleasa
Affiliation: Pharmaceutical Technology Department, Analytical Chemistry Department
„Carol Davila” University of Medicine and Pharmacy, Bucharest,
Abstract: Dexamethasone, Dxm, is frequently used as an immunosuppressive to treat a broad range of autoimmune and inflammatory disorders, most of them occur locally and near the body surface. So, topical application could offer the advantage of delivering a drug directly to the affected surface, but barrier properties of skin limit the dexamethasone’s permeability. Transdermal penetration could be improved by drug complexation with cyclodextrins. The purpose of this paper is to analyze the formation of inclusion complexes of the drug with β-cyclodextrin and hydroxypropyl-β-cyclodextrin. Investigations were made by phase solubility diagrams and scanning electron microscopy in order to evaluate: the formation of 1:1 molar ratios, complexation efficiency, improved solubility, morphology and homogeneity samples. Huguchi and Connors method and fractal analysis were applied to compare the performances of the two final binary solutions.
Keywords: dexamethasone, cyclodextrins, inclusion complex, phase solubility diagram, fractal analysis
References: Aleem O., Kuchekar B., Pore Y., Late S., Effect of β- cyclodextrin and hydroxypropyl-β-cyclodextrin complexation on physicochemical properties and antimicrobial activity of cefdinir, J.Pharm.Biomed. Anal, 47, 535-540, 2008,
Armatas G.S., Kolonia K.M., and Pomonis P.J., Morphometry of porous solids: lacunarity, fractal dimensions, connectivity, and some topological similarities with neurons. Langmuir, 18, 26, 3552-3558, 2002,
Bibby D. C., Davies N . M., and Tucker I. G., Mechanisms by which cyclodextrins modify drug release from polymeric drug delivery systems. Int. J. Pharm. 197:1–11, 2000,
Brewster M.E., Loftsson T., Cyclodextrins as pharmaceutical solubilizers, Adv.Drug Deliv.Rev., 59, 645-666, 2007,
Higuchi T., Connors K.A., Phase-solubility techniques, Adv.Anal.Chem.Instr., 4, 117-122, 1965, Cázares D. J., Fernández C.B., Pascual A. C., Rondero A., G., Guerrero D.Q., Castellano L., Merino V., Kalia V., Transdermal iontophoresis of dexamethasone sodium phosphate in vitro and in vivo: Effect of experimental parameters and skin type on drug stability and transport kinetics, European Journal of Pharmaceutics and Biopharmaceutics Volume 75, Issue 2, June, p 173-178, 2010, Davies E.R., Handbook of Texture Analysis, Chapter 1— Introduction to texture analysis, Imperial College Press, London, UK, 2008,
Dong P., Lacunarity analysis of raster datasets and 1d, 2d, and 3d point patterns. Computers and Geosciences, 35, 2009,
Fansook P., Ritthidej G., Ueda H., Stefánsson E., Loftsson T., γCD/HPγCD Mixtures as Solubilizer: Solid- State Characterization and Sample Dexamethasone Eye Drop Suspension, J Pharm Pharmaceut Sci
(www.cspsCanada.org) 13(3), p 336-350, 2010,
Faula D., Oliveira D., Tedesco A.C., Bentley M. V., Enhancing effect of modified beta-cyclodextrins on in vitro skin permeation of estradiol, Revista Brasileira de Ciências Farmacêuticas, Brazilian Journal of Pharmaceutical Sciences, vol. 43, n. 1, jan./mar., 2007,
Gavriloaia M.R, Creţu E.A, Lupuleasa D, Infrared studies on the thermal efect of topical glucocorticoids/ cyclodextrins complexes, ACTA MEDICA MARISIENSIS, Volume 56, Supplement 2, The XIVth National Congress of Pharmacy from Romania, Tîrgu-Mureş, October 13th – 16th, 2010,
Official publication of the University of Medicine and Pharmacy of Târgu Mureş, ISSN 2068-3324, pag. 100, 2010.
Hagiwara T., Kumagai H., and Nakamura K., Fractal analysis of aggregates in heat-induced BSA gels. Food Hydrocolloids, 12, 1, p 1756-1763, 1998,
Karperien A., FracLac advanced user’s manual, Charles Sturt University, Australia, 2004,Lee T., Atkins,M. S., A new approach to measure border irregularity for melanocytic lesions, Medical Imaging 2000: Image Processing, Proceedings of SPIE Vol. 3979, p 250-256, 2000,
Luo L., and Lin H., Lacunarity and fractal analyses of soil macropores and preferential transport using micro-X-ray computed tomography. Vadose Zone Journal, 8, p 233-241, 2009,
Ozkan Y., Atay T., Dikmen N., Işimer A., Aboul-Enein H.Y., Improvement of water solubility and in vitro dissolution rate of gliclazide by complexation with β –cyclodextrin, Pharm. Acta Helv., 74, Apr;74(4):365-70. 2000,
Sonka M., Hlavac V., Boyle R., Texture, in: Image Processing, Analysis, and Machine Vision, third ed., Thomson Learning, Ontario, Canada, 2008,
Stella V. J., Rajewski R. A., Cyclodextrins: Their future in drug formulation and delivery, Pharm. Res., May; 14(5): 556-5671997:
Vianna R. F. L., Bentley M. V., Ribeiro L. B. G et al. Formation of cyclodextrin inclusion complexes with corticosteroids: their characterization and stability. Int. J. Pharm. V. 167, p.205-213, 1998.
*Correspondence: Mariuca-Roxana GAVRILOAIA*, „Carol Davila” University of Medicine and Pharmacy, Bucharest, Faculty of Pharmacy, 6 Traian Vuia St., 020956, Bucharest, Romania; e-mail: roxana21g@yahoo.com.