Estimation of genotoxic potential of carbendazim in fenugreek

Estimation of genotoxic potential of carbendazim in fenugreek

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Title: Estimation of genotoxic potential of carbendazim in fenugreek
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Article_Title: Estimation of genotoxic potential of carbendazim in fenugreek
Authors: Elena Truta1*, Gabriela Capraru1, Maria Magdalena Zamfirache2, Maria Asaftei3, Constantin Toma2, Zenovia Olteanu2, Lacramioara Ivanescu2
Affiliation: 1 Biological Research Institute of Iasi
2 “Al. I. Cuza” University of Iaşi, Faculty of Biology
3 Clinical Hospital “I.C. Parhon” of Iaşi
Abstract: Because the studies on pesticide effects evidenced a diversity of chromosomal aberrations, in relation to the analysed class of compounds, we considered necessary to test the influence of carbendazim fungicide on genetic material of fenugreek (Trigonella foenum graecum L.), which can constitute, as other plant species, monitoring systems in the evaluation of possible genetic risk of the pesticide use. Mitotic index, the frequency of cell division phases, the types and incidence of ana-telophase aberrations, and the types of metaphase abnormalities were studied. The mitotic index was lower in all carbendazim treated variants; the most numerous ana-telophase aberrations and metaphase abnormalities were noted in 0.5% and 1.0% carbendazim treated variants. The effect of carbendazim on height growth of plantlets in early ontogenetic phases was also investigated – an inverse relation between concentration and plantlets’ height was registered.
Keywords: carbendazim, chromosome aberrations, mitotic index, Trigonella foenum graecum L.
References: Advisory Committee on Pesticides, Evaluation on carbendazim, evaluation of fully approved or provisionally approved products, No. 58. Ministry of Agriculture, Fisheries and Food, 1992.
Căpraru G, Băra IC, Băra II, Cîmpeanu M, Maxim VE, Numerical and morphostructural characteristics of Trigonella foenum graecum (2n=16) mitotic chromosomes. Proceedings of 4th Conference on Medicinal and Aromatic Plants of South-East European Countries, Iaşi, Romania, 28th – 31 May 2006, Alma Mater Publ. House, 53–57, 2006.
El-Ghamery AA, El-Nahas AI, Mansour MM, The action of atrazine herbicide as an inhibitor of cell division on chromosomes and nucleic acid content in root meristems of Allium cepa and Vicia faba. Cytologia, 65, 277–287, 2000.
Gamborg OL, Wetter LR, Plant tissue culture methods. National Research Council, Saskatoon, Canada, 1975.
Grant WF, Chromosome aberrations in plants as a monitoring system. Environmental Health Perspectives, 27, 37–43, 1978.
Hicks B, Generic pesticides – the products and markets. Agrow reports, PJB publications, 1998.
Klasterska I, Natrajan AT, Ramel C, An interpretation of the origin of subchromatid aberrations and chromosome aberrations and chromosome stickiness as a category of chromatid aberrations. Hereditas, 83:153–162, 1976.
Levan A, The effect of colchicine on root mitoses in Allium. Hereditas, 24, 147, 1968.
Permjit K, Grover IS, Cytological effects of some organophosphorus pesticides. Meiotic effects. Cytologia, 50, 199–211, 1985.
Quian Y, Transformation and expression of the resistance gene to carbendazim into Trichoderma harzianum. Resistant Pest Management, 6, 8–12, 1996.
Schneidermann MH, Dewey WC, Highfield DP, Inhibition of DNA synthesis in synchronized Chinese hamster cell treated in G1 with cycloheximide. Experimental Cell Research 67:147–155, 1971.
Singh P, Srivastava AK, Singh AK, Cell cycle stage specific application of cypermethrin and carbendazim to assess the genotoxicity in somatic cells of Hordeum vulgare L. Bulletin of Environmental Contamination and toxicology (Bull Environ Contam Toxicol), 81, 258–261, 2008.
Storey WB, Jordan LS, Mann JD, Carbamate herbicides – new tools for cytological studies. California Agriculture, 22(8), 12–13, 1968.
Sudhakar R, Ninge Gowda KN, Venu G, Mitotic abnormalities induced by silk dyeing industry effluents in the cells of Allium cepa. Cytologia, 66, 235–239, 2001.
Tomkins DJ, Grants WF, Comparative cytological effects of pesticides: menazon metrobromuron and tetrachloroisophthalonitrile in Hordeum vulgare and Tradescantia. Canadian Journal of Genetics and Cytology, 14, 245–256, 1972.
Yenjerla M, Cox C, Wilson L, Jordan MA, Carbendazim inhibits cancer cell proliferation by suppressing microtubule dynamics, The Journal of Pharmacology and Experimental Therapeutics (JPET), 328(2), 390-398, 2009.
http://www.pfaf.org/database/plants.php?trigonella+foenum-graecum
Read_full_article: pdf/20-2010/20-2-2010/SU20-2-10Truta.pdf
Correspondence: Elena Truta, Department of Cell Biology, Biological Research Institute, Lascar Catargiu nr. 47, 700107 Iasi, Romania, email: trutaelena@yahoo.com

Read full article
Article Title: Estimation of genotoxic potential of carbendazim in fenugreek
Authors: Elena Truta1*, Gabriela Capraru1, Maria Magdalena Zamfirache2, Maria Asaftei3, Constantin Toma2, Zenovia Olteanu2, Lacramioara Ivanescu2
Affiliation: 1 Biological Research Institute of Iasi
2 “Al. I. Cuza” University of Iaşi, Faculty of Biology
3 Clinical Hospital “I.C. Parhon” of Iaşi
Abstract: Because the studies on pesticide effects evidenced a diversity of chromosomal aberrations, in relation to the analysed class of compounds, we considered necessary to test the influence of carbendazim fungicide on genetic material of fenugreek (Trigonella foenum graecum L.), which can constitute, as other plant species, monitoring systems in the evaluation of possible genetic risk of the pesticide use. Mitotic index, the frequency of cell division phases, the types and incidence of ana-telophase aberrations, and the types of metaphase abnormalities were studied. The mitotic index was lower in all carbendazim treated variants; the most numerous ana-telophase aberrations and metaphase abnormalities were noted in 0.5% and 1.0% carbendazim treated variants. The effect of carbendazim on height growth of plantlets in early ontogenetic phases was also investigated – an inverse relation between concentration and plantlets’ height was registered.
Keywords: carbendazim, chromosome aberrations, mitotic index, Trigonella foenum graecum L.
References: Advisory Committee on Pesticides, Evaluation on carbendazim, evaluation of fully approved or provisionally approved products, No. 58. Ministry of Agriculture, Fisheries and Food, 1992.
Căpraru G, Băra IC, Băra II, Cîmpeanu M, Maxim VE, Numerical and morphostructural characteristics of Trigonella foenum graecum (2n=16) mitotic chromosomes. Proceedings of 4th Conference on Medicinal and Aromatic Plants of South-East European Countries, Iaşi, Romania, 28th – 31 May 2006, Alma Mater Publ. House, 53–57, 2006.
El-Ghamery AA, El-Nahas AI, Mansour MM, The action of atrazine herbicide as an inhibitor of cell division on chromosomes and nucleic acid content in root meristems of Allium cepa and Vicia faba. Cytologia, 65, 277–287, 2000.
Gamborg OL, Wetter LR, Plant tissue culture methods. National Research Council, Saskatoon, Canada, 1975.
Grant WF, Chromosome aberrations in plants as a monitoring system. Environmental Health Perspectives, 27, 37–43, 1978.
Hicks B, Generic pesticides – the products and markets. Agrow reports, PJB publications, 1998.
Klasterska I, Natrajan AT, Ramel C, An interpretation of the origin of subchromatid aberrations and chromosome aberrations and chromosome stickiness as a category of chromatid aberrations. Hereditas, 83:153–162, 1976.
Levan A, The effect of colchicine on root mitoses in Allium. Hereditas, 24, 147, 1968.
Permjit K, Grover IS, Cytological effects of some organophosphorus pesticides. Meiotic effects. Cytologia, 50, 199–211, 1985.
Quian Y, Transformation and expression of the resistance gene to carbendazim into Trichoderma harzianum. Resistant Pest Management, 6, 8–12, 1996.
Schneidermann MH, Dewey WC, Highfield DP, Inhibition of DNA synthesis in synchronized Chinese hamster cell treated in G1 with cycloheximide. Experimental Cell Research 67:147–155, 1971.
Singh P, Srivastava AK, Singh AK, Cell cycle stage specific application of cypermethrin and carbendazim to assess the genotoxicity in somatic cells of Hordeum vulgare L. Bulletin of Environmental Contamination and toxicology (Bull Environ Contam Toxicol), 81, 258–261, 2008.
Storey WB, Jordan LS, Mann JD, Carbamate herbicides – new tools for cytological studies. California Agriculture, 22(8), 12–13, 1968.
Sudhakar R, Ninge Gowda KN, Venu G, Mitotic abnormalities induced by silk dyeing industry effluents in the cells of Allium cepa. Cytologia, 66, 235–239, 2001.
Tomkins DJ, Grants WF, Comparative cytological effects of pesticides: menazon metrobromuron and tetrachloroisophthalonitrile in Hordeum vulgare and Tradescantia. Canadian Journal of Genetics and Cytology, 14, 245–256, 1972.
Yenjerla M, Cox C, Wilson L, Jordan MA, Carbendazim inhibits cancer cell proliferation by suppressing microtubule dynamics, The Journal of Pharmacology and Experimental Therapeutics (JPET), 328(2), 390-398, 2009.
http://www.pfaf.org/database/plants.php?trigonella+foenum-graecum
*Correspondence: Elena Truta, Department of Cell Biology, Biological Research Institute, Lascar Catargiu nr. 47, 700107 Iasi, Romania, email: trutaelena@yahoo.com