Leptin mediates the lipopolysaccharide-increased pulmonary permeability in rats

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Article Title:	Leptin mediates the lipopolysaccharide-increased pulmonary permeability in rats
Authors:	Bogdan Cristian Petrescu, Bogdan Gurzu, Roxana Irina Iancu, Anca Indrei, Irina Luciana Dumitriu, Liliana Chelaru, Simona Mihaela Slatineanu, Gheorghe Petrescu, Marcel Costuleanu
Affiliation:	University of Medicine and Pharmacy “Gr. T. Popa” Iasi, Romania
Abstract:	The aim of our study was represented by the leptin involvement as a mediator of lipopolysaccharide (LPS)-increased pulmonary permeability in rats. Pretreatment with aminoguanidine (20 mg/kg i.p), 2-aminopurine (20 mg/kg i.p.) and subjecting to food deprivation (48 h, with water ad libitum) reduced the LPS-induced vascular leak, measured as Evans blue extravasation in lung tissue, by 41.70%, 21.39% and, respectively, 52.92%, when compared to LPS (10µg/kg, 100µl aliquots) administered alone. In addition, leptin alone induced Evans blue leakage that represents 74.38% from that induced by LPS. Since the inhibitory effects of 2-aminopurine are with 59.34% higher than that induced by aminoguanidine on leptin, we were able to conclude that leptin is at least partially mediating the LPS-increased pulmonary permeability in rats. Lung concentrations of leptin might increase as an early event of LPS challenge. Furthermore, leptin effects might be partially realized through NO production after leptin receptors activation.
Keywords:	leptin, lipopolysaccharide, nitric oxide, pulmonary permeability, rat
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*Correspondence:	Marcel Costuleanu, University of Medicine and Pharmacy “Gr. T. Popa” Iasi, Faculty of Dental Medicine, Department of General and Oro-Maxillo-Facial Pathology, 16, University Str., 700115, Iasi, Romania, Tel. +40-745-589050, Fax. +40-232-211820, email: mcostuleanu@yahoo.com