Dosage of some organic compounds in the leaves of the Sedum telephium ssp. maximum l. plants harvested from nature compared with plants obtained on the culture of Murashige – Skoog supplemented with growth regulator


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Article Title: Dosage of some organic compounds in the leaves of the Sedum telephium ssp. maximum l. plants harvested from nature compared with plants obtained on the culture of Murashige – Skoog supplemented with growth regulator
Authors: Ardelean M., Cosma-Cachiță D., Turcuș V.
Affiliation: Department of Plant Biotechnology, Institute of Life Sciences, Vasile Goldiș Western University of Arad
Abstract: In the near future, the medicinal species of Sedum telephium ssp. maximum L. studied by us, would become general interest for the biotechnical procedures of obtaining phytopharmaceuticals sublayers, because of their secondary products of metabolism that they synthetize. The purpose of the present study was to determine the content of organic compounds of the leaves harvested from nature and the vitro-leaves taken from the vitro-plants grown on culture of Murashige – Skoog (1962) which had been treated with various growth regulators. The organic compounds determined have been: ascorbic acid, total proteins, total carbohydrates, total polyphenols and anthocyans. The absorptivity of extracts obtained has been calculated with a UV/Vis T60U spectrophotometer. The biochemical determinations carried out on the vegetal material have indicated that the quantities of organic compounds were significantly increased in the samples taken from the in vitro-plants of Sedum telephium ssp. maximum L., cultivated on the culture of Murashige – Skoog, especially in those with additions of kinetin and naphthaleneacetic acid, in comparison with the results gathered from the leaves harvested from plants grown in nature
Keywords: Keywords: Sedum telephium ssp. maximum L., proteins, carbohydrates, polyphenols, antocyans, growth regulators
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*Correspondence: Mirela Ardelean PhD., Vasile Goldiş Western University of Arad, Bulevardul Revoluţiei, 94, Arad, Romania, email: mirela.ardelean1@yahoo.com