ER-negative breast carcinomas – An immunophenotypical study
Authors: Diana NARITA1, Natalia CIREAP2, Razvan ILINA2, Dragos IZVERNARIU3, Aurel ARDELEAN4, George PRIBAC4
Affiliation: 1 Department of Biochemistry, University of Medicine and Pharmacy „Victor Babes” Timisoara, Romania; 2 Department of Oncological Surgery, University of Medicine and Pharmacy „Victor Babes” Timisoara, Romania; 3 Department of Histology, University of Medicine and Pharmacy „Victor Babes” Timisoara, Romania; 4 Department of Cell and Molecular Biology, “Vasile Goldis” Western University, Faculty of Medicine, Pharmacy and Dental Medicine, Arad, Romania
ABSTRACT. Our aim was to study the morphological and immunohistochemical characteristics of a series of breast carcinomas, in comparative manner, between ER-negative and ER-positive tumours and to establish immunophenotypes of the ER-negative breast carcinomas. We established the morphological diagnosis and grade of 80-selected breast carcinomas on haematoxylin-eosin samples; additional sections were immunostained for AR, PSA, ER and PR, HER2/neu. The presence of lymphoid infiltrate, comedo-type necrosis, fibrosis and bizarre tumour giant cells were the most common morphological features in the ER-negative tumours. The apocrine character and the clear cells changes, squamoid cells changes, the medullary character and the adenoid cystic pattern were found only in the ER-negative carcinomas. We observed a significant correlation between the expression of ER and the grade of differentiation, PR, AR, PSA and HER2/neu expression; the majority of ER-negative carcinomas were poorly differentiated, PR-, and PSA-negative, but AR- and HER2/neu positive. The most frequent ER-negative immunophenotype in our study was the triple-negative phenotype (ER-/PR-/HER-) and the majority of these cases were AR-positive. All HER2/neu positive cases were also AR-positive. Our study supports the emerging studies that suggest a cross talk between steroids receptors, HER2/neu and PSA in breast carcinomas.
Keywords: estrogen receptor, androgen receptor, HER2/neu, prostate-specific antigen